N1-003iC4
UGOTSAi004-B
General
Cell Line |
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| hPSCreg name | UGOTSAi004-B |
| Cite as: | UGOTSAi004-B (RRID:CVCL_C1X8) |
| Alternative name(s) |
N1-003iC4
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
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| Last update | 21st December 2022 |
| User feedback | |
Provider |
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| Generator | The Sahlgrenska Academy at University of Gothenburg (UGOTSA) |
External Databases |
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| Cellosaurus | CVCL_C1X8 |
| Wikidata | Q114313158 |
| BioSamples | SAMEA112259049 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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| Sex | female |
| Age of donor (at collection) | 75-79 |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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| Is the medical history available upon request? | No |
| Is clinical information available? | NO |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
No
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA112184262 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes |
| Confirm that consent was obtained by a qualified professional | Yes |
| Has the donor agreed to be re-contacted? | Unknown |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| Does consent pertain to a specific research project? | Yes |
| Details on restriction to research project | To study disease mechanisms related to neurodegeneration and to test drug candidates in the relevant field. |
| Does consent permit unforeseen future research, without further consent? | No |
| Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? | No |
| Does consent expressly permit storage of donated embryo/tissue for an unlimited time? | Yes |
| Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? | Yes |
| Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
Does consent permit research by | |
| an academic institution? | Yes |
| Does consent expressly permit collection of genetic information? | Yes |
| Does consent expressly permit storage of genetic information? | Yes |
| Does consent prevent dissemination of genetic information? | No |
| How may genetic information associated with the cell line be accessed? | No information |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? | No |
| Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? | Yes |
| Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? | Yes |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Regionala etikprövningsnämnden i Göteborg (EPN) |
| Approval number | 731-14 |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | Yes |
| Name of accrediting authority involved? | Regionala etikprövningsnämnden i Göteborg (EPN) |
| Approval number | 731-14 |
| Do you have obligations to third parties in regard to the use of the cell line? | No |
| Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? | No |
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
hIPSC Derivation
General |
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| Source cell type |
Any skin fibroblast that is part of some dermis.
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| Source cell origin |
Either of the fleshy mounds in the rear pelvic area of the human body formed by the gluteal muscles.
Synonyms
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| Age of donor (at collection) | 75-79 |
| Collected in | 2018 |
| Passage number reprogrammed | 2 |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | ReproRNA™-OKSGM |
| Is reprogramming vector detectable? |
Unknown |
Vector free reprogramming |
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Other |
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| Selection criteria for clones | Clones consisting of iPSCs only, large enough to be picked manually and with a sharp edge towards other cells were selected. |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
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| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ Plus
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| SSEA-1 |
No |
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| SSEA-4 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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Differentiation Potency
In vitro directed differentiation
Morphology
003iC4 trilineage ICC summary.pdf
Trilineage ICC summary endoderm mesoderm ectoderm
In vitro directed differentiation
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
Normal karyotype; 46, XX
Passage number: 12
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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