SFC091-04-03

General

Cell Line

hPSCreg name STBCi034-A
Cite as:
STBCi034-A (RRID:CVCL_RC06)
Alternative name(s)
SFC091-04-03
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
STBCi034-B
(SFC091-04-01)
Donor diseases:
migraine disorder
STBCi046-A
(SFC021-03-01)
Donor diseases:
migraine disorder
STBCi046-B
(SFC021-03-02)
Donor diseases:
migraine disorder
STBCi253-A
(SFC184-01-04)
Donor diseases:
migraine disorder
STBCi255-A
(SFC183-01-07)
Donor diseases:
migraine disorder
STBCi257-A
(SFC185-01-11)
Donor diseases:
migraine disorder
STBCi260-A
(SFC279-03-02)
Donor diseases:
migraine disorder
STBCi261-A
(SFC186-01-07)
Donor diseases:
migraine disorder
STBCi262-A
(SFC271-03-06)
Donor diseases:
migraine disorder
STBCi263-A
(SFC268-03-02)
Donor's gene variants:
ATP1A2
Donor diseases:
migraine disorder
STBCi055-C
(SFC026-04-04)
Donor diseases:
migraine disorder
STBCi027-A
(SFC017-03-01)
Donor diseases:
migraine disorder
STBCi027-B
(SFC017-03-02)
Donor diseases:
migraine disorder
STBCi028-A
(SFC018-03-01)
Donor diseases:
migraine disorder
STBCi028-B
(SFC018-03-02)
Donor diseases:
migraine disorder
STBCi028-C
(SFC018-03-04)
Donor diseases:
migraine disorder
STBCi029-A
(SFC020-03-02)
Donor diseases:
migraine disorder
STBCi029-B
(SFC020-03-03)
Donor diseases:
migraine disorder
STBCi030-A
(SFC023-03-24)
Donor diseases:
migraine disorder
STBCi030-B
(SFC023-03-43)
Donor diseases:
migraine disorder
STBCi031-A
(SFC024-04-02)
Donor diseases:
migraine disorder
STBCi031-B
(SFC024-04-03)
Donor diseases:
migraine disorder
STBCi031-C
(SFC024-04-01)
Donor diseases:
migraine disorder
STBCi141-A
(SFC103-03-01)
Donor diseases:
migraine disorder
STBCi055-A
(SFC026-04-03)
Donor diseases:
migraine disorder
STBCi055-B
(SFC026-04-10)
Donor diseases:
migraine disorder
STBCi311-A
(BPC941-03-12)
Donor diseases:
migraine disorder
LUBi001-A
(PGRN-8310, PGRN8310, RCi195, RCFB58 c12.5)
Donor's gene variants:
GRN, GRN, GRN, GRN
Donor diseases:
Frontotemporal dementia
LUBi001-B
(PGRN-8310, RCFB58 c4.4, PGRN8310, RCi194)
Donor's gene variants:
GRN, GRN, GRN, GRN
Donor diseases:
Frontotemporal dementia
LUBi001-C
(PGRN-8310, RCFB58 c3.7, RCi200, PGRN8310)
Donor's gene variants:
GRN, GRN, GRN, GRN
Donor diseases:
Frontotemporal dementia
UCLi004-A
(RCi173, RCFB60c6)
Donor's gene variants:
C9orf72, C9orf72
Donor diseases:
Amyotrophic lateral sclerosis
Frontotemporal dementia
UCLi004-B
(RCFB60c7, RCi177)
Donor's gene variants:
C9orf72, C9orf72
Donor diseases:
Amyotrophic lateral sclerosis
Frontotemporal dementia
UCLi004-C
(RCi172, RCFB60c2)
Donor's gene variants:
C9orf72, C9orf72
Donor diseases:
Amyotrophic lateral sclerosis
Frontotemporal dementia
STBCi071-C
(SFC057-07-02)
Donor diseases:
Alzheimer disease
STBCi090-B
(SFC867-04-12)
Donor diseases:
Parkinson disease
CHDIi002-A
(#2c3, CHDI-90002150)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi006-A
(#6c7, CHDI-90002154)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi008-A
(#8c3, CHDI-90002156)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi011-A
(#11c2, CHDI-90002159)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi017-A
(#17c1, CHDI-90002165)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi018-A
(#18c1, CHDI-90002166)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi019-A
(#19c9, CHDI-90002167)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi021-A
(#21c5, CHDI-90002169)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi028-A
(#28c3, CHDI-90002176)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
STBCi009-A
(SFC801-03-01)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi009-B
(SFC801-03-02)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi009-C
(SFC801-03-03)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
CHDIi035-A
(#108c5, CHDI-90002183)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
STBCi010-A
(SFC802-03-06)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
CHDIi036-A
(#110c5, CHDI-90002184)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
Last update 24th March 2023
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator StemBANCC (STBC)
Distributors

External Databases

BioSamples SAMEA104493685
EBiSC STBCi034-A
Cellosaurus CVCL_RC06
Wikidata Q54956445

General Information

Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male
Age of donor (at collection) 20-24

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • migraine
  • migraine disorder
  • migraine headache
  • migraine variant
  • migraine with or without aura
show more synonyms
Genetic variants

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104493686

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Contact information / weblink zameel.cader@ndcn.ox.ac.uk
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? No
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? NRES Committee South Central - Hampshire A
Approval number 13/SC/0179
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? NRES Committee South Central - Hampshire A
Approval number 13/SC/0179
Do you have obligations to third parties in regard to the use of the cell line? Yes
Please describe: DNA sequencing can only be performed for research into donors specified condition. Material shall not be sold, transplanted into any human being or used to create egg or sperm cells (gametes) or embryos. The material shall not be used for direct exploitation. For the purposes of this, Direct exploitation means to develop for commericalization or to commercialize the Material.
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Age of donor (at collection) 20-24

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
TRA 1-60
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 10

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No abnormalities detected
Passage number: 10
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)