CHES-1

General#

Cell Line

hPSCreg Name SYSUe003-A
Alternative name(s)
CHES-1
Cell line type Human embryonic stem cell (hESC)
Last update 22nd May 2019
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Provider

Generator Sun Yat-sen University (SYSU)
Derivation country China

External Databases

Cellosaurus CVCL_C808
CLO CLO_0100465

General Information

* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics#

Alternatives to consent

hESC Derivation#

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
No
Other hESC source: IVF
ZP removal technique Enzymatic
Cell isolation Immunosurgery

Culture Conditions#

Feeder cells mouse embryonic fibroblast cells
Cellfinder Ont Id: EFO_0004040
Passage method Mechanically
Medium Other medium:
Base medium: DMEM
Main protein source: Fetal bovine serum
Supplements
L-Glutamine
LIF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
SSEA-1
No
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Passage number: 43

Other Genotyping (Cell Line)