SYSUi007-A
General
Cell Line |
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hPSCreg name | SYSUi007-A |
Cite as: | SYSUi007-A (RRID:CVCL_D0M2) |
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines |
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Last update | 28th August 2023 |
User feedback | |
Provider |
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Generator | Sun Yat-sen University (SYSU) |
Owner | Sun Yat-Sen University, center for stem cell biology and tissue engineering (SYSUSC) |
Distributors | |
Derivation country | China |
External Databases |
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BioSamples | SAMEA114266905 |
Cellosaurus | CVCL_D0M2 |
Wikidata | Q123033583 |
General Information |
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* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Donor Information
General Donor Information |
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Sex | male |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
Yes
46,XY
Karyotyping method:
G-Banding
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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BioSamples | SAMEA114266906 |
Ethics
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
Was the consent voluntarily given? | Yes |
Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes |
Alternatives to consent are available? | No |
Is there other documentation provided to the donor for consenting purposes? | No |
Confirm that consent was obtained by a qualified professional | Yes |
Has the donor agreed to be re-contacted? | Unknown |
Has the donor been informed about how her/his data will be protected? | Yes |
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
Does consent permit research by | |
an academic institution? | Yes |
Does consent expressly permit collection of genetic information? | Yes |
How may genetic information associated with the cell line be accessed? | Controlled Access |
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
Name of accrediting authority involved? | 中山医学院医学伦理委员会 |
Approval number | 中山医医伦 (2022)第 014号 |
Is there an MTA available for the cell line? | No |
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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Source cell type |
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
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Reprogramming method |
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Vector type | Non-integrating |
Vector | Sendai virus |
Is reprogramming vector detectable? |
No |
Methods used |
PCR
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Vector free reprogramming |
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Other |
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Derived under xeno-free conditions |
Unknown |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
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Medium |
mTeSR™ 1
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
NANOG |
Yes |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
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POU5F1 (OCT-4) |
Yes |
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Differentiation Potency
Microbiology / Virus Screening |
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Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46, XY
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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