UBTi001-A-1

PEB-AL#6CD43Rep#3

General

iPSC Line

hPSCreg name UBTi001-A-1
Cite as:
UBTi001-A-1
Alternative name(s)
PEB-AL#6CD43Rep#3
iPSC line type Human induced pluripotent stem cell (hiPSC)
Similar iPSC lines No similar lines found.
Last update 20th August 2026
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Provider

Generator Medical University of Graz (UBT)

External iPSC Databases

BioSamples SAMEA117796709

General iPSC Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

iPSC Genetic Modification

Genetic modifications not related to a disease
SPN/CD43 (target)
Gene knock-in
16p11.2
GFP
CD43-GFP Reporter
CRISPR-associated (CRISPR/Cas) System

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA14181372

Ethics

Also have a look at the ethics information for the parental line UBTi001-A .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

iPSC Derivation

General

The source cell information can be found in the parental cell line UBTi001-A.

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

iPSC Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Mechanically
Medium Other medium:
Base medium: StemMACS™ iPS-Brew XF, human
Main protein source:
Serum concentration: %
Supplements
StemMACS™ iPS-Brew Supplement XF (50×), human %
Has Rock inhibitor (Y27632) been used at passage previously with this iPSC line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this iPSC line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this iPSC line?
Yes

iPSC Characterisation

Analysis of undifferentiated iPSCs
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
OCT4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SSEA-4
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
Alpha-SMA
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
OTX2
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (iPSC Line)

Has the iPSC line karyotype been analysed?
Yes
46, XY
Karyotyping method: G-Banding

Other Genotyping (iPSC Line)