NP0114-2C
UKKi026-B
General#
Cell Line |
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hPSCreg Name | UKKi026-B |
Alternative name(s) |
NP0114-2C
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Last update | 8th December 2017 |
User feedback | |
Provider |
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Generator | Klinikum der Universität zu Köln (UKK) |
Owner | Institute for Neurophysiology, Medical Faculty |
Distributors | |
Derivation country | Germany |
External Databases |
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BioSamples | SAMEA104132932 |
Cellosaurus | CVCL_LD46 |
EBiSC | UKKi026-B |
ECACC | 66540595 |
General Information |
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Projects | |
Is the cell line available in principle? | Available, without restrictions |
Donor Information#
General Donor Information |
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Sex | male |
Ethnicity | chinese |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
No
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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Other cell lines of this donor | |
External Databases (Donor) |
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BioSamples | SAMEA104132931 |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
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Source cell type |
peripheral blood mononuclear cellA leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.
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Age of donor (at collection) | 25-29 |
Collected in | 2016 |
Passage number reprogrammed | 0 |
Reprogramming method |
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Vector type | Non-integrating |
Vector | Sendai virus |
Genes | |
Is reprogramming vector detectable? |
No |
Methods used |
PCR
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Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
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Type of used vector free reprogramming factor(s) |
None
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Other |
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Selection criteria for clones | morphology |
Derived under xeno-free conditions |
Yes |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Surface coating | Vitronectin | ||||||
Feeder cells |
No |
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Passage method |
Enzyme-free cell dissociation
EDTA
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O2 Concentration | 20 % | ||||||
CO2 Concentration | 5 % | ||||||
Medium |
Other medium:
Base medium: StemFlex
Main protein source: Serum concentration: % Supplements
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
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Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
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Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
Differentiation Potency
In vitro directed differentiation
Marker | Expressed |
BRACHYURY |
Yes |
In vitro directed differentiation
Marker | Expressed |
Nestin |
Yes |
Microbiology / Virus Screening |
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HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
No larger chromosomal aberrations to be reported.
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Other Genotyping (Cell Line) |
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