NP0114-5E

General#

Cell Line

hPSCreg Name UKKi026-C
Alternative name(s)
NP0114-5E
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
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Provider

Generator Klinikum der Universität zu Köln (UKK)
Distributors
Derivation country Germany

External Databases

BioSamples SAMEA104132933
Cellosaurus CVCL_LD47
ECACC 66540596
EBiSC UKKi026-C

General Information

Is the cell line available in principle? Available, without restrictions

Donor Information#

General Donor Information

Sex male
Ethnicity chinese

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104132931

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
peripheral blood mononuclear cell
A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.
Age of donor (at collection) 25-29
Collected in 2016
Passage number reprogrammed 0

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones morphology
Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemFlex
Main protein source:
Serum concentration: %
Supplements
Essentail 8 supplement 50x 10 ml
Essentail 8 Flex supplement 50x 10 ml
StemFlex supplement 10x 50 ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
SOX2
Yes
SSEA-1
Yes
SSEA-4
Yes
TRA 1-80
Yes
OCT 4
Yes
FOXD3
Yes
DNMT3b
Yes
CD30
Yes
Differentiation Potency
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17
Yes
Morphology
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY
Yes
Morphology
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin
Yes
Morphology

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
no larger aberrations to be reported. Chr10: small mosaicism on long arm
Karyotyping method: Molecular karyotyping by SNP array

Other Genotyping (Cell Line)