SK310 CL5
The cell line is not validated yet.
UKWi009-A-1
General
iPSC Line |
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| hPSCreg name | UKWi009-A-1 |
| Cite as: | UKWi009-A-1 |
| Alternative name(s) |
SK310 CL5
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| iPSC line type | Human induced pluripotent stem cell (hiPSC) |
| Similar iPSC lines |
UKWi009-A (SK310 CL1) |
| Last update | 14th November 2025 |
| User feedback | |
Provider |
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| Generator | Universitätsklinikum Würzburg (UKW) |
External iPSC Databases |
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| BioSamples | SAMEA120322962 |
General iPSC Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
iPSC Genetic Modification
| Genetic modifications not related to a disease |
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Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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External Databases (Donor) |
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| BioSamples | SAMEA120322953 |
Ethics
Also have a look at the ethics information for the parental line
UKWi009-A
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
iPSC Derivation
General |
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The source cell information can be found in the parental cell line
UKWi009-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
| Methods used |
RT-PCR
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Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
iPSC Culture Conditions
| Surface coating | Matrigel/Geltrex | ||||||
| Feeder cells |
irradiated Mouse Embryonic Fibroblasts |
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| O2 Concentration | 20 % | ||||||
| CO2 Concentration | 5 % | ||||||
| Medium |
Other medium:
Base medium: StemMACS™ PSC-Brew XF, human
Main protein source: Serum concentration: 0 % Supplements
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| Has Rock inhibitor (Y27632) been used at passage previously with this iPSC line? | Yes |
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| Has Rock inhibitor (Y27632) been used at cryo previously with this iPSC line? | No |
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| Has Rock inhibitor (Y27632) been used at thaw previously with this iPSC line? | Yes |
iPSC Characterisation
Analysis of undifferentiated iPSCs
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SSEA-4 |
Yes |
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Morphology pictures
Differentiation Potency
In vitro directed differentiation
Protocol or reference
In vitro directed differentiation
Protocol or reference
In vitro directed differentiation
Protocol or reference
Genotyping
Karyotyping (iPSC Line) |
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Other Genotyping (iPSC Line) |
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