General#

Cell Line

hPSCreg Name UNEWi018-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
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Provider

Generator University of Newcastle (UNEW)
Distributors
Derivation country United Kingdom

External Databases

BioSamples SAMEA3142165
Cellosaurus CVCL_II94
ECACC 66540012
EBiSC UNEWi018-A

General Information

Projects
Is the cell line available in principle? Available, without restrictions

Donor Information#

General Donor Information

Sex male
Ethnicity caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Aplastic anemia
The donor is affected.
Disease associated phenotypes
  • pancytopenia and hypocellular bone marrow
Family history No
Is the medical history available upon request? No
Is clinical information available? Idiopathic severe aplastic anaemia. Responded to rabbit Lymphoglobulin but then relapsed. Now in partial recovery following treatment with lymphocyte immune globulin

External Databases (Donor)

BioSamples SAMEA3142155

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Age of donor (at collection) 10-14

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Matrigel/Geltrex
O2 Concentration 95 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-1
No
SSEA-4
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
trisomy chromosome 3
Karyotyping method: CytoSNP

Other Genotyping (Cell Line)