F324 5.8

General#

Cell Line

hPSCreg Name UNEWi025-A
Alternative name(s)
F324 5.8
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 22nd May 2019
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Provider

Generator University of Newcastle (UNEW)
Owner Institute of Genetic Medicine
Distributors
Derivation country United Kingdom

External Databases

BioSamples SAMEA4447907
Cellosaurus CVCL_IT94
ECACC 66540328
EBiSC UNEWi025-A

General Information

Projects
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: allowed
Commercial: allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 70-74

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
age-related macular degeneration
The donor is a carrier of a disease-associated mutation.
Synonyms
  • Macular Dystrophy
  • Degeneration, Macular
  • Age Related Maculopathies
  • MACULAR DEGENERATION NOS
  • Age related maculopathy
  • Age Related Macular Degeneration
  • Unspecified senile macular degeneration
  • Maculopathies, Age Related
  • MACULOPATHY AGE RELAT
  • AAMD - Age related macular degeneration
  • Maculopathies, Age-Related
  • Macular Dystrophies
  • ARMD
  • Amended
  • Dystrophies, Macular
  • Age-Related Maculopathy
  • Senile macular degeneration
  • SMD - Senile macular degeneration
  • Macular degeneration (senile) of retina, unspecified
  • Macular Degeneration, Age-Related
  • Degeneration, Age-Related Macular
  • Macular degeneration (disorder)
  • Macular Degenerations
  • Degenerations, Age-Related Macular
  • AGE RELAT MACULOPATHIES
  • Macular Degenerations, Age-Related
  • Maculopathy, Age-Related
  • AMD - Age-related macular degeneration
  • Maculopathy, Age Related
  • Dystrophy, Macular
  • Degenerations, Macular
  • Senile macular retinal degeneration
  • Age-related macular degeneration (disorder)
  • Age related macular degeneration (disorder) [Ambiguous]
  • AGE RELAT MACULOPATHY
  • MACULOPATHIES AGE RELAT
  • Age-Related Macular Degenerations
  • Age-Related Maculopathies
  • Senile macular degeneration of retina
  • ARMD - Age-related macular degeneration
  • Macular Degeneration
  • macular degeneration, age-related
  • AMD
  • age-related macular degeneration
  • age related maculopathy
  • age related Maculopathies
show more synonyms
Genetic variants
CFH (target)
1q31.3
NM_000186.3:c.1204C>T
NP_000177.2:p.His402Tyr NC_000001.11:g.19669010
Heterozygous
SCV000038294.5

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA4447906

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? No
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: Dr David Steel
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? Yes
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent pertain to a specific research project? Yes
Details on restriction to research project Research use restricted to Retinal Degeneration and Dystrophies
Does consent permit unforeseen future research, without further consent? Yes
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? No
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
Does consent expressly permit collection of genetic information? Yes
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? Yes
Policy for access to genetic information Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? No
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? North East - Newcastle & North Tyneside 1 Research Ethics Committee
Approval number 11/NE/0294
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? North East - Newcastle & North Tyneside 1 Research Ethics Committee
Approval number 11/NE/0294
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 70-74
Passage number reprogrammed P4

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
TRA 1-60
Yes
SSEA-1
No
SSEA-4
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
ACTA2
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
TUBB3
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: P12

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No clinically significant imbalance was detected
Karyotyping method: Molecular karyotyping by SNP array

Other Genotyping (Cell Line)