The cell line was withdrawn.
(only basic data is shown)


Cell Line

hPSCreg Name WAe009-A-14
Cell line type Human embryonic stem cell (hESC)
Last update 22nd May 2019
User feedback
Written by Zhiyu Zeng on 2018-12-05 03:06:40  
We have generated a homozygous ATP2A2 knockout human embryonic stem cell line H9-ATP2A2-/- .H9 was transfected with Cas9 vector(Px459,Addgene,62988) which expresses a specific sgRNA sequence targeting for the exon1 of ATP2A2. After electroporation, cells were seeded on a 6-well, Matrigel precoated overnight plate and treated with 0.3ug/mL puromycin in E8 medium. Anti-puromycin clones were emerged after 4 days of electroporation. 15 clones were picked and maintained in E8 medium. DNA was extracted for genotype confirming using PCR sequencing. Clone number 4 was confirmed by homozygous deleting 1 “A” base pair. For further characterization, this clone was expanded. H9-ATP2A2-/- grows as a clone size and expresses stem cell pluripotency hallmarks like OCT-4, SSEA4, processing a normal karyotype and the ability to differentiate into all three germ layers in vitro.

Login to share your feedback, experiences or results with the research community.


Generator The First Affiliated Hospital of Guangxi Medical University (FAHGMU)
Owner The First Affiliated Hospital of Guangxi Medical University (FAHGMU)

External Databases

BioSamples SAMEA5573702
Cellosaurus CVCL_UM38

General Information

* Is the cell line readily obtainable for third parties?
Research: allowed
Clinical: allowed
Commercial: allowed
Subclone of