WAe009-A-3X

The cell line is not validated yet.

General

Cell Line

hPSCreg name WAe009-A-3X
Cite as:
WAe009-A-3X
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 3rd June 2026
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Provider

Generator Beijing Anzhen Hospital, Capital Medical University (BAH)

External Databases

BioSamples SAMEA122769259

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Genetic Modification

Disease/phenotype related modifications
Synonyms
  • NDD
Genetic modifications
Gene knock-out
15q15.3
FRMD5
CRISPR-associated (CRISPR/Cas) System
Genetic modifications not related to a disease
Gene knock-out
15q15.3
FRMD5
CRISPR-associated (CRISPR/Cas) System

Donor Information

General Donor Information

Sex female
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7768918

Ethics

Also have a look at the ethics information for the parental line WAe009-A .
Is there an MTA available for the cell line? No
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hESC Derivation

The source cell information can be found in the parental cell line WAe009-A.

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells hESC
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium Essential 8™ Flex
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
HAND1
Unknown
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
Nestin
Unknown
Morphology

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX

Other Genotyping (Cell Line)