WISCe001-A-1

General#

Cell Line

hPSCreg Name WISCe001-A-1
Cell line type Human embryonic stem cell (hESC)
Last update 22nd May 2019
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Provider

Generator University of Wisconsin (WISC)
Derivation country United States

External Databases

CLO CLO_0102883

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

Ethics#

Also have a look at the ethics information for the parental line WISCe001-A .
Alternatives to consent
Name of accrediting authority involved?
Approval number

hESC Derivation#

Embryo stage Blastula with ICM and Trophoblast
ZP removal technique Enzymatic
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions#

Surface coating Matrigel/Geltrex
Passage method Mechanically
O2 Concentration 5 %
CO2 Concentration 10 %
Medium mTeSR™ 1
Supplements
bFGF
GABA
LiCl

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
ZFP42 (REX-1)
Yes
TERT
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
47XY,+12

Other Genotyping (Cell Line)

Genetic Modification#