WISCe001-A-1
General
Cell Line |
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| hPSCreg name | WISCe001-A-1 |
| Cite as: | WISCe001-A-1 |
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
WISCe001-A (WA15) |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
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| Generator |
University of Wisconsin (WISC)
Contact:
Madison - Thomson Lab |
| Derivation country | United States |
External Databases |
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General Information |
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| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics
Also have a look at the ethics information for the parental line
WISCe001-A
.
| Alternatives to consent | |
| Name of accrediting authority involved? | |
| Approval number |
hESC Derivation
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The source cell information can be found in the parental cell line
WISCe001-A.
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Culture Conditions
| Surface coating | Matrigel/Geltrex | ||||||||||||
| Passage method | Mechanically | ||||||||||||
| O2 Concentration | 5 % | ||||||||||||
| CO2 Concentration | 10 % | ||||||||||||
| Medium |
mTeSR™ 1
Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| ZFP42 (REX-1) |
Yes |
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| TERT |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
47XY,+12
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Other Genotyping (Cell Line) |
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