HPSI0513i-debk_7

General#

Cell Line

hPSCreg Name WTSIi055-B
Alternative name(s)
HPSI0513i-debk_7
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 9th May 2018
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Provider

Generator Wellcome Trust Sanger Institute (WTSI)
Distributors

External Databases

BioSamples SAMEA2398966
ECACC 77650225
HipSci HPSI0513i-debk_7
Cellosaurus CVCL_AF38

General Information

Publications
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2398311
HipSci HPSI-debk

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Feeder cells Mouse embryo fibroblast (MEF) feeder cells
Passage method Enzymatically
Collagenase and Dispase
CO2 Concentration 5 %
Medium Other medium:
Base medium:
Main protein source: Knock-out serum replacement

Characterisation#

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
20.42 1.209

Certificate of Analysis

Is there a certificate of analysis available?
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0513i-debk_7
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
RNA-seq
https://ega-archive.org/datasets/EGAD00001003529
Splice-aware STAR alignment
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001139
Text file with probe intensities
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001139
Array signal intensity data
Genotyping array
https://ega-archive.org/datasets/EGAD00010001147
Imputed and phased genotypes
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003514
Imputed and phased genotypes
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003514
Raw sequencing reads
Genotyping array
https://ega-archive.org/datasets/EGAD00010001147
Array signal intensity data
Genotyping array
https://ega-archive.org/datasets/EGAD00010001147
Genotyping array calls
RNA-seq
https://ega-archive.org/datasets/EGAD00001003529
Abundances of transcripts
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003514
mpileup variant calls
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003514
BWA alignment