HPSI0114i-joxm_1
WTSIi074-A
General#
Cell Line |
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hPSCreg Name | WTSIi074-A |
Alternative name(s) |
HPSI0114i-joxm_1
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Last update | 9th May 2018 |
User feedback | |
Provider |
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Generator | Wellcome Trust Sanger Institute (WTSI) |
Distributors | |
Derivation country | United Kingdom |
External Databases |
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BioSamples | SAMEA2547637 |
HipSci | HPSI0114i-joxm_1 |
Cellosaurus | CVCL_AE28 |
ECACC | 77650047 |
EBiSC | WTSIi074-A |
General Information |
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Publications | |
Projects | |
Is the cell line available in principle? | Available with restrictions: Available for non-commercial research |
Donor Information#
General Donor Information |
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Sex | female |
Ethnicity | White - Other |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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External Databases (Donor) |
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BioSamples | SAMEA2398839 |
HipSci | HPSI-joxm |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
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Source cell type |
fibroblastA connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
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Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
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Age of donor (at collection) | 45-49 |
Collected in | 2014 |
Source cell line vendor | Cambridge BioResource |
Reprogramming method |
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Vector type | Non-integrating |
Vector | Sendai virus |
Genes | |
Notes on reprogramming vector detection | CytoTune 1 |
Vector free reprogramming |
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Other |
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Selection criteria for clones | Morphology |
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Surface coating | Vitronectin |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
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CO2 Concentration | 5 % |
Medium |
TeSR™ E8™
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Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
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SOX2 |
Yes |
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NANOG |
Yes |
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Pluripotency Score | Novelty Score | Link to microarray data |
23.796 | 1.335 | http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/ |
Report
HPSI-joxm.pluritest.pluripotency_score.20161010.png
pluripotency image
HPSI-joxm.pluritest.novelty_score.20161010.png
novelty image
Microbiology / Virus Screening |
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HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Certificate of Analysis |
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Is there a certificate of analysis available? |
Yes
Passage:
31
|
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
No
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Other Genotyping (Cell Line) |
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Is there genome-wide genotyping or functional data available? |
Yes
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266794
cnv
http://www.hipsci.org/lines/#/lines/HPSI0114i-joxm_1 Number of regions different from primary tissue: 1; Length of differences from primary tissue: 2
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/ Text file with probe intensities |
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