HPSI0114i-joxm_1
WTSIi074-A
General#
Cell Line |
|
| hPSCreg Name | WTSIi074-A |
| Alternative name(s) |
HPSI0114i-joxm_1
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Last update | 9th May 2018 |
| User feedback | |
Provider |
|
| Generator | Wellcome Trust Sanger Institute (WTSI) |
| Distributors | |
| Derivation country | United Kingdom |
External Databases |
|
| BioSamples | SAMEA2547637 |
| HipSci | HPSI0114i-joxm_1 |
| Cellosaurus | CVCL_AE28 |
| ECACC | 77650047 |
| EBiSC | WTSIi074-A |
General Information |
|
| Publications | |
| Projects | |
| Is the cell line available in principle? | Available with restrictions: Available for non-commercial research |
Donor Information#
General Donor Information |
|
| Sex | female |
| Ethnicity | White - Other |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
External Databases (Donor) |
|
| BioSamples | SAMEA2398839 |
| HipSci | HPSI-joxm |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
|
| Source cell type |
fibroblastA connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
|
| Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
|
| Age of donor (at collection) | 45-49 |
| Collected in | 2014 |
| Source cell line vendor | Cambridge BioResource |
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Notes on reprogramming vector detection | CytoTune 1 |
Vector free reprogramming |
|
Other |
|
| Selection criteria for clones | Morphology |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
| Surface coating | Vitronectin |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| CO2 Concentration | 5 % |
| Medium |
TeSR™ E8™
|
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
|
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| SOX2 |
Yes |
|
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| NANOG |
Yes |
|
| Pluripotency Score | Novelty Score | Link to microarray data |
| 23.796 | 1.335 | http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/ |
Report
HPSI-joxm.pluritest.pluripotency_score.20161010.png
pluripotency image
HPSI-joxm.pluritest.novelty_score.20161010.png
novelty image
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Certificate of Analysis |
|
| Is there a certificate of analysis available? |
Yes
Passage:
31
|
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
No
|
Other Genotyping (Cell Line) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266794
cnv
http://www.hipsci.org/lines/#/lines/HPSI0114i-joxm_1 Number of regions different from primary tissue: 1; Length of differences from primary tissue: 2
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/ Text file with probe intensities |
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