HPSI1213i-xuja_2

General#

Cell Line

hPSCreg Name WTSIi083-A
Alternative name(s)
HPSI1213i-xuja_2
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 9th May 2018
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Wellcome Trust Sanger Institute (WTSI)
Distributors
Derivation country United Kingdom

External Databases

BioSamples SAMEA2494988
HipSci HPSI1213i-xuja_2
Cellosaurus CVCL_AI23
ECACC 77650087
EBiSC WTSIi083-A

General Information

Publications
Projects
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex female
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA2397810
HipSci HPSI-xuja

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 45-49
Collected in 2014
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score Link to microarray data
34.569 1.348 http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 36

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI1213i-xuja_2
Number of regions different from primary tissue: 1; Length of differences from primary tissue: 1
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR914302
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR914338
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1901045
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127593
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122925
mpileup variant calls
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127358
Imputed and phased genotypes
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR947057
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ123058
Splice-aware STAR alignment
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR947065
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ447977
GATK haplotype calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ267002
Abundances of transcripts
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122991
Imputed and phased genotypes
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities