HPSI0913i-gedo_33

General#

Cell Line

hPSCreg Name WTSIi113-B
Alternative name(s)
HPSI0913i-gedo_33
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 9th May 2018
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Provider

Generator Wellcome Trust Sanger Institute (WTSI)
Distributors
Derivation country United Kingdom

External Databases

BioSamples SAMEA2398982
HipSci HPSI0913i-gedo_33
Cellosaurus CVCL_AH38
ECACC 77650167

General Information

Projects
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2399211
HipSci HPSI-gedo

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score Link to microarray data
28.058 1.98 https://www.ebi.ac.uk/ega/datasets/EGAD00010000775

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0913i-gedo_33
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003514
BWA alignment
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003514
mpileup variant calls
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003514
Raw sequencing reads
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001139
Array signal intensity data
Genotyping array
https://ega-archive.org/datasets/EGAD00010001147
Imputed and phased genotypes
Genotyping array
https://ega-archive.org/datasets/EGAD00010001147
Genotyping array calls
RNA-seq
https://ega-archive.org/datasets/EGAD00001003529
Abundances of transcripts
Expression array
https://ega-archive.org/datasets/EGAD00010001143
Array signal intensity data
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003514
Imputed and phased genotypes
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001139
Text file with probe intensities
Genotyping array
https://ega-archive.org/datasets/EGAD00010001147
Array signal intensity data
Expression array
https://ega-archive.org/datasets/EGAD00010001143
GenomeStudio text file
RNA-seq
https://ega-archive.org/datasets/EGAD00001003529
Splice-aware STAR alignment