HPSI0813i-fpdk_2
WTSIi206-A
General
Cell Line |
|
| hPSCreg name | WTSIi206-A |
| Cite as: | WTSIi206-A (RRID:CVCL_AH02) |
| Alternative name(s) |
HPSI0813i-fpdk_2
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 20th January 2021 |
| User feedback | |
Provider |
|
| Generator | Wellcome Sanger Institute (WTSI) |
| Distributors | |
| Derivation country | United Kingdom |
External Databases |
|
| BioSamples | SAMEA2201448 |
| HipSci | HPSI0813i-fpdk_2 |
| Cellosaurus | CVCL_AH02 |
| Wikidata | Q54891374 |
General Information |
|
| Publications | |
| Projects | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
Donor Information
General Donor Information |
|
| Sex | male |
| Age of donor (at collection) | 40-44 |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
Exome sequencing
https://ega-archive.org/datasets/EGAD00001001932
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001139
SNP typing array
https://ega-archive.org/datasets/EGAD00010001147 |
External Databases (Donor) |
|
| BioSamples | SAMEA2444542 |
| HipSci | HPSI-ffdk |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | Yes |
| Please provide the contact information | Willem Ouwehand, Cambridge BioResource |
| Is there other documentation provided to the donor for consenting purposes? | No |
| Confirm that consent was obtained by a qualified professional | Yes |
| Has the donor agreed to be re-contacted? | No |
| Has the donor been informed about how her/his data will be protected? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| Does consent pertain to a specific research project? | No |
| Does consent permit unforeseen future research, without further consent? | Yes |
| Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? | No |
| Does consent expressly prevent development of commercial products? | No |
| Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? | No |
| Does consent expressly permit storage of donated embryo/tissue for an unlimited time? | Yes |
| Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
Does consent permit research by | |
| an academic institution? | Yes |
| a public organisation? | Yes |
| a non-profit company? | Yes |
| a for-profit corporation? | Yes |
| Does consent expressly permit collection of genetic information? | Yes |
| Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? | Yes |
| How may genetic information associated with the cell line be accessed? | No information |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? | No |
| Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | NRES Committee Yorkshire & The Humber - Leeds West |
| Approval number | 15/YH/0391 |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | Yes |
| Name of accrediting authority involved? | NRES Committee Yorkshire & The Humber - Leeds West |
| Approval number | 15/YH/0391 |
| Do you have obligations to third parties in regard to the use of the cell line? | No |
| Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
hIPSC Derivation
General |
|
| Source cell type |
A leukocyte with a single non-segmented nucleus in the mature form.
Synonyms
|
| Source cell origin |
A fluid that is composed of blood plasma and erythrocytes.
Synonyms
|
| Age of donor (at collection) | 40-44 |
| Collected in | 2013 |
| Source cell line vendor | Cambridge BioResource |
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Notes on reprogramming vector detection | CytoTune 1 |
Vector free reprogramming |
|
Other |
|
| Selection criteria for clones | Morphology |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Feeder cells |
Mouse embryo fibroblast (MEF) feeder cells |
| Passage method |
Enzymatically
Collagenase and Dispase
|
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium:
Main protein source: Knock-out serum replacement Serum concentration: % |
Characterisation
Analysis of Undifferentiated Cells
| Pluripotency Score | Novelty Score | Link to microarray data |
| 37.797 | 1.705 | https://ega-archive.org/datasets/EGAD00010001143 |
Report
HPSI-ffdk.pluritest.pluripotency_score.20170510.png
pluripotency image
HPSI-ffdk.pluritest.novelty_score.20170510.png
novelty image
Transcriptome Characterisation
Differentiation Potency
In vitro spontaneous differentiation
| Marker | Expressed |
| Sox17 |
Yes |
Protocol or reference
IFforgermlayermarkershIPSCs1WTSIi206-A.jpg
Immunostaining
In vitro spontaneous differentiation
| Marker | Expressed |
| Brachyuri |
Yes |
Protocol or reference
IFforgermlayermarkershIPSCs1WTSIi206-A.jpg
Immunostaining
Neuroectoderm
In vitro spontaneous differentiation
| Marker | Expressed |
| Sox1 |
Unknown |
Protocol or reference
IFforgermlayermarkershIPSCs1WTSIi206-A.jpg
Immunestaining
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
No
|
Other Genotyping (Cell Line) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
Exome sequencing
https://ega-archive.org/datasets/EGAD00001001932
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001139
SNP typing array
https://ega-archive.org/datasets/EGAD00010001147
cnv
http://www.hipsci.org/lines/#/lines/HPSI0813i-fpdk_2 Number of regions different from primary tissue: 1; Length of differences from primary tissue: 4
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001139 Text file with probe intensities |
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