HPSI0513i-leeh_3

General

Cell Line

hPSCreg name WTSIi214-A
Cite as:
WTSIi214-A (RRID:CVCL_AF58)
Alternative name(s)
HPSI0513i-leeh_3
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
WTSIi214-B
(HPSI0513i-leeh_2)
Last update 22nd May 2019
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Provider

Generator Wellcome Sanger Institute (WTSI)
Distributors

External Databases

HipSci HPSI0513i-leeh_3
BioSamples SAMEA2398586
Cellosaurus CVCL_AF58
Wikidata Q54890835

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2398951

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Provide contact information of the holder of the original Donor Information Sheet:
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? No
Contact information / weblink
Alternatives to consent
Alternative consent approval number
Is there other documentation provided to the donor for consenting purposes? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Details on restriction to research project
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed?
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Please describe how access is provided:
Contact data, institution, or website:
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? East of England- Cambridge Central Research Ethics Committee.
Approval number
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? No
Name of accrediting authority involved?
Approval number
Please describe:
Further constraints on use
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Constraints for use or distribution

hIPSC Derivation

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Feeder cells Mouse embryo fibroblast (MEF) feeder cells
Passage method Enzymatically
Collagenase and Dispase
CO2 Concentration 5 %
Medium Other medium:
Base medium:
Main protein source: Knock-out serum replacement

Characterisation

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
48.413 1.348

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 29

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0513i-leeh_3
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Genotyping array
https://ega-archive.org/datasets/EGAD00010001147
Array signal intensity data
Genotyping array
https://ega-archive.org/datasets/EGAD00010001147
Genotyping array calls
RNA-seq
https://ega-archive.org/datasets/EGAD00001003529
Abundances of transcripts
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003514
Imputed and phased genotypes
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003514
Raw sequencing reads
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001139
Text file with probe intensities
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003514
mpileup variant calls
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001139
Array signal intensity data
Genotyping array
https://ega-archive.org/datasets/EGAD00010001147
Imputed and phased genotypes
RNA-seq
https://ega-archive.org/datasets/EGAD00001003529
Splice-aware STAR alignment
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003514
BWA alignment