HPSI0213i-koun_4
WTSIi248-B
General
Cell Line |
|
| hPSCreg name | WTSIi248-B |
| Cite as: | WTSIi248-B (RRID:CVCL_AE44) |
| Alternative name(s) |
HPSI0213i-koun_4
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
WTSIi248-A (HPSI0213i-koun_2) |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
|
| Generator | Wellcome Sanger Institute (WTSI) |
External Databases |
|
| BioSamples | SAMEA2399269 |
| HipSci | HPSI0213i-koun_4 |
| Cellosaurus | CVCL_AE44 |
| Wikidata | Q54890521 |
General Information |
|
| Projects | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
Donor Information
General Donor Information |
|
| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
Donor Relations |
|
| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA2399272 |
Ethics
| Alternatives to consent | |
| How may genetic information associated with the cell line be accessed? |
hIPSC Derivation
General |
|
| Source cell type |
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
|
| Source cell origin |
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
|
| Source cell line vendor | Cambridge BioResource |
Reprogramming method |
|
Vector free reprogramming |
|
Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Feeder cells |
Yes |
| Passage method |
Enzymatically
Collagenase and Dispase
|
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium:
|
Characterisation
Analysis of Undifferentiated Cells
| Pluripotency Score | Novelty Score | |
| 55.155 | 1.409 |
Report
HPSI-koun.pluritest.pluripotency_score.20161010.png
pluripotency image
HPSI-koun.pluritest.novelty_score.20161010.png
novelty image
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
No
|
Other Genotyping (Cell Line) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0213i-koun_4 Number of regions different from primary tissue: 1; Length of differences from primary tissue: 1 |
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