HPSI0115i-vazt_1

General#

Cell Line

hPSCreg Name WTSIi252-B
Alternative name(s)
HPSI0115i-vazt_1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 14th May 2018
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Wellcome Trust Sanger Institute (WTSI)
Distributors

External Databases

BioSamples SAMEA4089843
ECACC 77650425
HipSci HPSI0115i-vazt_1
Cellosaurus CVCL_EE83

General Information

Publications
Projects
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex male
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3182893

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 55-59
Collected in 2015
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Notes on reprogramming vector detection CytoTune 2

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
19.568 1.262

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0115i-vazt_1
Number of regions different from primary tissue: 1; Length of differences from primary tissue: 3
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ369256
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376875
mpileup variant calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376308
Splice-aware STAR alignment
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861032
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859880
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1743804
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1724715
Raw sequencing reads
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860840
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860648
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860744
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1680163
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860360
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859976
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861224
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861128
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ368964
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1680195
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1680227
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376699
Imputed and phased genotypes
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860936
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1680259
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376523
BWA alignment
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376167
Abundances of transcripts
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860552
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860456
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ448237
GATK haplotype calls