HPSI0514i-letw_1

General#

Cell Line

hPSCreg Name WTSIi253-B
Alternative name(s)
HPSI0514i-letw_1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 14th May 2018
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Provider

Generator Wellcome Trust Sanger Institute (WTSI)
Distributors

External Databases

BioSamples SAMEA3962566
ECACC 77650394
HipSci HPSI0514i-letw_1
Cellosaurus CVCL_EE94

General Information

Publications
Projects
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex male
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2547897

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 70-74
Collected in 2015
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Notes on reprogramming vector detection CytoTune 2

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
36.448 1.572

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0514i-letw_1
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376211
Splice-aware STAR alignment
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1743811
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1680267
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376574
Imputed and phased genotypes
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376070
Abundances of transcripts
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ369105
Genotyping array calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1724723
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1680235
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ447965
GATK haplotype calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1680203
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1680171
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376750
mpileup variant calls
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ368813
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376398
BWA alignment
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861362
Raw sequencing reads