HPSI0914i-jejf_2

General#

Cell Line

hPSCreg Name WTSIi254-A
Alternative name(s)
HPSI0914i-jejf_2
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 22nd May 2019
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Provider

Generator Wellcome Trust Sanger Institute (WTSI)
Distributors

External Databases

HipSci HPSI0914i-jejf_2
BioSamples SAMEA3854309
ECACC 77650570
Cellosaurus CVCL_AK14

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 50-54
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2784228

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? No
If you do not hold the SIGNED Donor Consent Form, do you know who does? No
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? Yes
Alternatives to consent
Is there other documentation provided to the donor for consenting purposes? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? East of England- Cambridge Central Research Ethics Committee.
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? No

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 50-54
Collected in 2015
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Notes on reprogramming vector detection CytoTune 2

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
34.186 1.221

Certificate of Analysis

Is there a certificate of analysis available?
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0914i-jejf_2
Number of regions different from primary tissue: 3; Length of differences from primary tissue: 1
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376068
Abundances of transcripts
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1540864
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ368764
Genotyping array calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1555137
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1555089
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376209
Splice-aware STAR alignment
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859414
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859318
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860182
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859510
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860086
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859798
Raw sequencing reads
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376748
mpileup variant calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1837072
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376572
Imputed and phased genotypes
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859030
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1594758
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860278
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859702
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859606
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ369056
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1555074
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1837063
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376396
BWA alignment
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859222
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859126
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ447956
GATK haplotype calls