HPSI0115i-hecn_3

General#

Cell Line

hPSCreg Name WTSIi269-B
Alternative name(s)
HPSI0115i-hecn_3
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 14th May 2018
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Wellcome Trust Sanger Institute (WTSI)
Distributors

External Databases

BioSamples SAMEA3853173
ECACC 77650412
HipSci HPSI0115i-hecn_3
Cellosaurus CVCL_EE77

General Information

Publications
Projects
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex female
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3180222

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 60-64
Collected in 2015
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Notes on reprogramming vector detection CytoTune 2

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
7.024 1.868

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0115i-hecn_3
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1680213
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1680181
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376479
BWA alignment
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860486
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860006
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860582
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859910
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ369225
Genotyping array calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1743792
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860966
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ448142
GATK haplotype calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1724703
Raw sequencing reads
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376128
Abundances of transcripts
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376655
Imputed and phased genotypes
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861254
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860390
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861158
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860678
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860870
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860774
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ368933
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1680245
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1680149
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376831
mpileup variant calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376269
Splice-aware STAR alignment
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861062
Raw sequencing reads