HPSI0714i-kute_4

General#

Cell Line

hPSCreg Name WTSIi300-B
Alternative name(s)
HPSI0714i-kute_4
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 14th May 2018
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Provider

Generator Wellcome Trust Sanger Institute (WTSI)
Distributors

External Databases

BioSamples SAMEA3974246
ECACC 77650426
HipSci HPSI0714i-kute_4
Cellosaurus CVCL_EF20

General Information

Publications
Projects
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex female
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2676423

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 25-29
Collected in 2015
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Notes on reprogramming vector detection CytoTune 2

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
25.893 1.37

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0714i-kute_4
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376359
BWA alignment
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ369092
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1680265
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1680169
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1724721
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376173
Splice-aware STAR alignment
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ447868
GATK haplotype calls
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ368800
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1680233
Raw sequencing reads
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376535
Imputed and phased genotypes
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861388
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1680201
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1823507
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376032
Abundances of transcripts
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376711
mpileup variant calls