HPSI0514i-naah_4

General#

Cell Line

hPSCreg Name WTSIi322-B
Alternative name(s)
HPSI0514i-naah_4
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 14th May 2018
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Provider

Generator Wellcome Trust Sanger Institute (WTSI)
Distributors

External Databases

BioSamples SAMEA3962619
ECACC 77650383
HipSci HPSI0514i-naah_4
Cellosaurus CVCL_EE99

General Information

Publications
Projects
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex male
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2519553

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 55-59
Collected in 2015
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Notes on reprogramming vector detection CytoTune 2

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
26.837 1.237

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0514i-naah_4
Number of regions different from primary tissue: 2; Length of differences from primary tissue: 1
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ368860
Genotyping array calls
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1680160
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376168
Splice-aware STAR alignment
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376524
Imputed and phased genotypes
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860588
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859916
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860684
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860780
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ369152
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1680192
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1680224
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861260
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861068
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861164
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ447843
GATK haplotype calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1743801
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1724712
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1680256
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376700
mpileup variant calls
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860876
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860972
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376027
Abundances of transcripts
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376348
BWA alignment
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860012
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860492
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860396
Raw sequencing reads