HPSI0715i-zaie_5

General#

Cell Line

hPSCreg Name WTSIi327-A
Alternative name(s)
HPSI0715i-zaie_5
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 9th May 2018
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Wellcome Trust Sanger Institute (WTSI)
Distributors

External Databases

HipSci HPSI0715i-zaie_5
BioSamples SAMEA3973871
ECACC 77650405
Cellosaurus CVCL_EF32

General Information

Publications
Projects
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex female
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3735552

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 70-74
Collected in 2015
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Notes on reprogramming vector detection CytoTune 2

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
33.718 1.289

Certificate of Analysis

Is there a certificate of analysis available?
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0715i-zaie_5
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1723714
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376234
Splice-aware STAR alignment
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376786
mpileup variant calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1724741
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1743828
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376434
BWA alignment
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ369070
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1702828
Raw sequencing reads
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861375
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ368778
Genotyping array calls
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ448035
GATK haplotype calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1723698
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376610
Imputed and phased genotypes
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376093
Abundances of transcripts