HPSI0513i-dovq_3

General

Cell Line

hPSCreg name WTSIi387-A
Cite as:
WTSIi387-A (RRID:CVCL_AF41)
Alternative name(s)
HPSI0513i-dovq_3
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 22nd May 2019
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Provider

Generator Wellcome Sanger Institute (WTSI)
Distributors

External Databases

HipSci HPSI0513i-dovq_3
BioSamples SAMEA2398899
Cellosaurus CVCL_AF41
Wikidata Q54890807

General Information

Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA2399169

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? No
Alternatives to consent
Is there other documentation provided to the donor for consenting purposes? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed?
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? East of England- Cambridge Central Research Ethics Committee.
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? No

hIPSC Derivation

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Feeder cells Mouse embryo fibroblast (MEF) feeder cells
Passage method Enzymatically
Collagenase and Dispase
CO2 Concentration 5 %
Medium Other medium:
Base medium:
Main protein source: Knock-out serum replacement

Characterisation

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
48.738 1.451

Certificate of Analysis

Is there a certificate of analysis available?
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0513i-dovq_3
Number of regions different from primary tissue: 39; Length of differences from primary tissue: 93
Genotyping array
https://ega-archive.org/datasets/EGAD00010001147
Imputed and phased genotypes
Genotyping array
https://ega-archive.org/datasets/EGAD00010001147
Genotyping array calls
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003514
BWA alignment
RNA-seq
https://ega-archive.org/datasets/EGAD00001003529
Abundances of transcripts
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003514
Raw sequencing reads
RNA-seq
https://ega-archive.org/datasets/EGAD00001003529
Splice-aware STAR alignment
Genotyping array
https://ega-archive.org/datasets/EGAD00010001147
Array signal intensity data
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003514
mpileup variant calls