HPSI0613i-qonr_1
WTSIi569-B
General#
Cell Line |
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| hPSCreg Name | WTSIi569-B |
| Alternative name(s) |
HPSI0613i-qonr_1
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
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| Generator | Wellcome Trust Sanger Institute (WTSI) |
| Distributors | |
External Databases |
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| BioSamples | SAMEA2398813 |
| HipSci | HPSI0613i-qonr_1 |
| Cellosaurus | CVCL_AG22 |
General Information |
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| Publications | |
| Projects | |
| * Is the cell line readily obtainable for third parties? |
Yes Research: allowed
Clinical: not allowed
Commercial: not allowed
|
Donor Information#
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA2399207 |
Ethics#
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
| Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? | No |
| If you do not hold the SIGNED Donor Consent Form, do you know who does? | No |
| If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? | Yes |
| Alternatives to consent |
| Is there other documentation provided to the donor for consenting purposes? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | East of England- Cambridge Central Research Ethics Committee. |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | No |
hIPSC Derivation#
General |
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| Source cell type |
fibroblastA connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
|
| Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
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| Source cell line vendor | Cambridge BioResource |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Notes on reprogramming vector detection | CytoTune 1 |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions#
| Feeder cells |
Mouse embryo fibroblast (MEF) feeder cells |
| Passage method |
Enzymatically
Collagenase and Dispase
|
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium:
Main protein source: Knock-out serum replacement |
Characterisation#
Analysis of Undifferentiated Cells
| Pluripotency Score | Novelty Score | |
| 40.116 | 1.331 |
Report
HPSI-qonr.pluritest.pluripotency_score.20161010.png
pluripotency image
HPSI-qonr.pluritest.novelty_score.20161010.png
novelty image
Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
No
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Other Genotyping (Cell Line) |
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| Is there genome-wide genotyping or functional data available? |
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0613i-qonr_1 Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0 |
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