HPSI0613i-qonr_1
WTSIi569-B
General#
Cell Line |
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| hPSCreg Name | WTSIi569-B |
| Alternative name(s) |
HPSI0613i-qonr_1
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Last update | 9th May 2018 |
| User feedback | |
Provider |
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| Generator | Wellcome Trust Sanger Institute (WTSI) |
| Distributors | |
External Databases |
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| BioSamples | SAMEA2398813 |
| HipSci | HPSI0613i-qonr_1 |
| Cellosaurus | CVCL_AG22 |
General Information |
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| Publications | |
| Projects | |
| Is the cell line available in principle? | Available with restrictions: Available for non-commercial research |
Donor Information#
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
|
Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA2399207 |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
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| Source cell type |
fibroblastA connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
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| Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
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| Source cell line vendor | Cambridge BioResource |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Notes on reprogramming vector detection | CytoTune 1 |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unkown |
| Derived under GMP? |
Unkown |
| Available as clinical grade? |
Unkown |
Culture Conditions#
| Feeder cells |
Mouse embryo fibroblast (MEF) feeder cells |
| Passage method |
Enzymatically
Collagenase and Dispase
|
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium:
Main protein source: Knock-out serum replacement |
Characterisation#
Analysis of Undifferentiated Cells
| Pluripotency Score | Novelty Score | |
| 40.116 | 1.331 |
Report
HPSI-qonr.pluritest.pluripotency_score.20161010.png
pluripotency image
HPSI-qonr.pluritest.novelty_score.20161010.png
novelty image
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
No
|
Other Genotyping (Cell Line) |
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| Is there genome-wide genotyping or functional data available? |
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0613i-qonr_1 Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0 |
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