Synonym of WTSIi558-B

HPSI0516i-oadp_5

General

Cell Line

hPSCreg name WTSIi572-A
Cite as:
WTSIi572-A (RRID:CVCL_IX54)
Alternative name(s)
HPSI0516i-oadp_5
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 6th October 2021
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Provider

Generator Wellcome Sanger Institute (WTSI)
Distributors

External Databases

BioSamples SAMEA4564046
HipSci HPSI0516i-oadp_5
Cellosaurus CVCL_IX54
Wikidata Q54890971

General Information

Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male
Ethnicity White British

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • Niikawa-Kuroki syndrome
  • Kabuki make-up syndrome

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA4452343

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? No
Is there other documentation provided to the donor for consenting purposes? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed?
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? North West 6 Research Ethics Committee
Approval number 11/H1003/3
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Collected in 2016
Source cell line vendor University of Manchester

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Notes on reprogramming vector detection CytoTune 2

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
40.353 1.576

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0516i-oadp_5
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003524
Raw sequencing reads
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003524
BWA alignment
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003524
mpileup variant calls
RNA-seq
https://ega-archive.org/datasets/EGAD00001003535
Abundances of transcripts
RNA-seq
https://ega-archive.org/datasets/EGAD00001003535
Splice-aware STAR alignment