hPSCreg®
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WTSIi677-A

Registration Summary:
Synonym of WTSIi676-B

HPSI0616i-heba_9

WTSIi677-A

General

Cell Line
hPSCreg name WTSIi677-A
Cite as:
WTSIi677-A (RRID:CVCL_LL75)
Alternative name(s)
HPSI0616i-heba_9
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 5th October 2021
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Provider
Generator Wellcome Sanger Institute (WTSI)
Distributors

External Databases
BioSamples SAMEA104011428
HipSci HPSI0616i-heba_9
Cellosaurus CVCL_LL75
Wikidata Q54891201

General Information
Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information
Sex female
Age of donor (at collection) 55-59
Ethnicity Any Other Asian Background - Seychellwa/Srilankan

Phenotype and Disease related information (Donor)
Diseases A disease was diagnosed.
Hematologic disease
Synonyms
  • Hematologic Diseases
  • blood disorder
  • hematopoietic system disease
  • hematologic disease
  • hematological disorder
  • disorder of hematopoietic system
  • hematopoietic system disease or disorder
  • hematopoietic disease
  • hematological system disease
  • disease of hematopoietic system
  • blood disease
  • haematological system disorder
  • hematologic and lymphocytic disorder
  • rare hematologic disease
  • disease of the blood and blood-forming organs
  • hematologic disorder
  • hematological system disorder
  • blood dyscrasia
  • haematological system disease
  • hematological disease
  • disease or disorder of hematopoietic system
  • disease of haematopoietic system
  • disease or disorder of haematopoietic system
  • disorder of haematopoietic system
  • haematological disease
  • haematological disorder
  • haematopoietic disease
  • haematopoietic system disease
  • haematopoietic system disease or disorder
  • haematological disorders and malignancies
  • hematological disorders and malignancies
show more synonyms

Donor Relations
Other cell lines of this donor

External Databases (Donor)
BioSamples SAMEA4459412

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? No
Is there other documentation provided to the donor for consenting purposes? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? East of England- Cambridge Central Research Ethics Committee.
Approval number
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
Source cell type
Fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 55-59
Collected in 2016
Source cell line vendor Cambridge BioResource

Reprogramming method
Vector type Non-integrating
Vector Sendai virus
Notes on reprogramming vector detection CytoTune 2

Vector free reprogramming

Other
Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
29.192 1.224

Certificate of Analysis
Is there a certificate of analysis available?
Yes
HPSI0616i-heba_9.CofA.20170905.pdf
Passage:

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)
Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0616i-heba_9