XCL-1

General#

Cell Line

hPSCreg Name XCLi001-A
Alternative name(s)
XCL-1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 7th May 2019
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Provider

Generator XCell Science Inc. (XCL)
Owner XCell Science Inc. (XCL)
Derivation country United States

External Databases

BioSamples SAMEA5728498

General Information

Publications
Is the cell line available in principle? Available with restrictions: Available for commercial research

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes
Is the medical history available upon request? No
Is clinical information available? No

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46,XY; Nonclonal Finding: 47,XY,+2,i(2)(p10),i(2)(q10)

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA5728498

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? No
If you do not hold the SIGNED Donor Consent Form, do you know who does? Yes
Contact information / weblink Confidential
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? Yes
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? No
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? Yes
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? No
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
Policy for access to genetic information Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? No
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? No
Does consent permit access to medical records of the donor? Yes
Please describe how access is provided: Refer to provided information
Does consent permit access to any other source of information about the clinical treatment or health of the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? No
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell type
Umbilical Cord Blood-derived Hematopoietic CD34-positive Progenitor Cells
A population of cryopreserved, ex vivo expanded and nicotinamide (NAM)-treated, CD34-positive hematopoietic progenitor cells (HPCs) derived from allogeneic, CD34+ cells isolated from human umbilical cord blood (UCB) that can be used during transplantation. CD34+ HPCs were isolated from human UCB mononuclear cells, and expanded ex vivo. Upon transplantation with the UCB-derived CD34+ HPCs, these cells can differentiate into a variety of cell types including fibroblasts, osteoblasts, chondrocytes, myocytes, adipocytes, and endothelial cells. Compared to bone marrow transplants, these HPCs have decreased risk of causing graft-versus host disease (GvHD), increased survival, and enhanced transplant and engraftment potential for any given patient as there is no need for a matched donor. Compared to untreated HPCs, treating the cells ex vivo with NAM increases the number of HPCs from UCB, enhances migration, bone marrow (BM) homing, engraftment and increases neutrophil and platelet recovery.
Synonyms
  • Ex Vivo-expanded Umbilical Cord Blood-derived Hematopoietic CD34-positive Progenitor Cells
  • NiCord
  • Umbilical Cord Blood-derived Hematopoietic CD34-positive Progenitor Cells
  • UCB-derived CD34+ HPCs
show more synonyms
Source cell origin
Umbilical Cord Blood
Blood present in the umbilical vessels at the time of delivery. If cryopreserved at birth, cord blood can serve as a source of hematopoietic progenitor cells for transplantation to a patient later diagnosed and treated for a hematopoietic disorder.
Synonyms
  • Cord Blood
  • Umbilical Cord Blood
  • CORD BLOOD
  • umbilical cord blood
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
Unkown

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Oct4
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Neuronal Stem Cell
Ont Id: CL_0000047
In vitro directed differentiation
Marker Expressed
Nestin
Yes
SOX1
Yes
Neuron
Ont Id: FMA_54527
In vitro directed differentiation
Marker Expressed
TUJ1
Yes
Dopaminergic Neuron
Ont Id: FMA_84787
In vitro directed differentiation
Marker Expressed
TUJ1
Yes
TH
Yes
Astrocyte
Ont Id: NCIT_C12477
In vitro directed differentiation
Marker Expressed
GFAP
Yes

Microbiology / Virus Screening

HIV 1 Negative
Hepatitis B Negative
Hepatitis C Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 12

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY; Nonclonal Finding: 47,XY,+2,i(2)(p10),i(2)(q10)

Other Genotyping (Cell Line)