General

Cell Line
hPSCreg name	BCHi028-A-1
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	BCHi028-A-1
Alternative name(s)	HNDS0171-01#A CNC2(+/-)
Cell line type	Human induced pluripotent stem cell (hiPSC)
Similar lines	BCHi028-A-2 (HNDS0171-01#A CC10(+/+)) Donor diseases: FOXG1 syndrome BCHi028-A (HNDS0171-01#A) Donor diseases: FOXG1 syndrome
Last update	8th March 2024
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Provider
Generator	Children's Hospital (BCH)
External Databases
BioSamples	SAMEA115390235
General Information
* Is the cell line readily obtainable for third parties?	Yes Research use: allowed Clinical use: not allowed Commercial use: not allowed
Subclone of	BCHi028-A

Donor Information

General Donor Information
Sex	male
Ethnicity	White
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. FOXG1 syndrome The donor is affected. Synonyms FOXG1-related epileptic-dyskinetic encephalopathy
Karyotyping (Donor)
Has the donor karyotype been analysed?	No
External Databases (Donor)
BioSamples	SAMEA115390170

Ethics

Also have a look at the ethics information for the parental line BCHi028-A .

Is there an MTA available for the cell line?	No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
The source cell information can be found in the parental cell line BCHi028-A.
Reprogramming method
Vector type	Non-integrating
Vector	Sendai virus
Vector free reprogramming
Other
Derived under xeno-free conditions	Unknown
Derived under GMP?	Unknown
Available as clinical grade?	Unknown

Culture Conditions

Surface coating	Matrigel/Geltrex
Feeder cells	No
Medium	Other medium: Base medium: Stemflex Main protein source: Bovine Albumine Serum (BSA) Serum concentration: 1 %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?	Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?	No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?	Yes

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line BCHi028-A .

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46, XY; no clonal abnormalities Karyotyping method: G-Banding
Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications

FOXG1 syndrome Synonyms FOXG1-related epileptic-dyskinetic encephalopathy Genetic modifications FOXG1 (target) Type of modification Variant Chromosome location Cytoband location: 14q12 Nucleotide sequence variant in HGVS format NM_005249.5: c.1094_1101dupACCGGCTG; p.V368TfsX20 Is the modification homozygous or heterozygous? Heterozygous