LATS1/2 KD
MUSIi012-A-9
General
iPSC Line |
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| hPSCreg name | MUSIi012-A-9 |
| Cite as: | MUSIi012-A-9 |
| Alternative name(s) |
LATS1/2 KD
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| iPSC line type | Human induced pluripotent stem cell (hiPSC) |
| Similar iPSC lines | No similar lines found. |
| Last update | 5th June 2026 |
| User feedback | |
Provider |
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| Generator | Faculty of Medicine Siriraj Hospital (MUSI) |
| Owner | Siriraj Center of Excellence for stem cell research |
| Distributors | |
| Derivation country | Thailand |
External iPSC Databases |
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| BioSamples | SAMEA122709837 |
General iPSC Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: not allowed
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| Subclone of | |
iPSC Genetic Modification
| Disease/phenotype related modifications |
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Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | Thai |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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| BioSamples | SAMEA6013991 |
Ethics
Also have a look at the ethics information for the parental line
MUSIi012-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
iPSC Derivation
General |
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The source cell information can be found in the parental cell line
MUSIi012-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
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| Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
iPSC Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| O2 Concentration | 5 % |
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium: Nutristem
Main protein source: Knock-out serum replacement Serum concentration: 0 % |
| Has Rock inhibitor (Y27632) been used at passage previously with this iPSC line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this iPSC line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this iPSC line? | No |
iPSC Characterisation
Analysis of undifferentiated iPSCs
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| NANOG |
Yes |
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| SOX2 |
Yes |
Morphology pictures
Figure B
Figure I, J, K
for pluripotentcy marker expression (I), and spontaneous differentiation (J and K).
Differentiation Potency
In vitro spontaneous differentiation
Morphology
Figure J, Alpha-fetoprotein
In vitro spontaneous differentiation
Morphology
Figure J, Beta-tubulin
Genotyping
Karyotyping (iPSC Line) |
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| Has the iPSC line karyotype been analysed? |
Yes
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Other Genotyping (iPSC Line) |
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