The cell line is not validated yet.
UCSFi001-A-1X
General
Cell Line |
|
| hPSCreg name | UCSFi001-A-1X |
| Cite as: | UCSFi001-A-1X |
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 23rd March 2026 |
| User feedback | |
Provider |
|
| Generator | University of Washington Institute for Stem Cell and Regenerative Medicine (ISCRM) |
| Owner | University of Washington Institute for Stem Cell and Regenerative Medicine (ISCRM) |
| Distributors | |
| Derivation country | United States |
External Databases |
|
| BioSamples | SAMEA120998336 |
General Information |
|
| * Is the cell line readily obtainable for third parties? |
Yes Cell line can only be used in: Not-for-profit research only use, obtainable after MTA
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
| Subclone of | |
Donor Information
General Donor Information |
|
| Sex | male |
| Ethnicity | Asian |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Family history | N/A |
| Is the medical history available upon request? | No |
| Is clinical information available? | Limited clinical information - EKG |
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
|
| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA5843920 |
Ethics
Also have a look at the ethics information for the parental line
UCSFi001-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | Addgene |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
|
|
The source cell information can be found in the parental cell line
UCSFi001-A.
|
|
| Passage number reprogrammed | NA |
Reprogramming method |
|
| Vector type | None |
Vector free reprogramming |
|
Other |
|
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ Plus
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
|||||
| SSEA-4 |
Yes |
Morphology pictures
WTC11 GCamp8f at day 4 after passaging (4X magnification - Bright field).
WTC11 GCamp8f at day 4 after passaging (10X magnification - Bright field).
Differentiation Potency
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
Genetic Modification
| Genetic modifications not related to a disease |
|
Login to share your feedback, experiences or results with the research community.