WBS02

The cell line is not validated yet.

General

Cell Line

hPSCreg name UMILi023-A
Cite as:
UMILi023-A (RRID:CVCL_YC86)
Alternative name(s)
WBS02
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
UMILi021-A
(WBS03)
Donor diseases:
Williams-Beuren syndrome
ESi059-A
([SWB] FiPS-4F-1-1)
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Williams syndrome
UMILi020-A
(WBS04)
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Williams-Beuren syndrome
UMILi022-A
(WBS01)
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Williams-Beuren syndrome
ESi059-B
([SWB] FiPS1-R4F-5)
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Williams syndrome
ESi060-A
([SWB] FiPS-4F-5-6)
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ESi060-B
([SWB] FiPS5-R4F-1)
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Williams syndrome
ESi100-A
(NW FiPS 10II.3-R4F-1)
Donor diseases:
Williams Syndrome
ESi068-A
(SWB FiPS 159-R4F-4)
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Williams-Beuren syndrome
ESi069-A
(SWB FiPS 344-R4F-2)
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UMILi013-A
(WVS01)
Donor diseases:
Weaver Syndrome
WTSIi485-B
(HPSI0614i-kecw_4)
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WTSIi399-B
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(HPSI0614i-laig_2)
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Bardet-Biedl syndrome
WTSIi033-A
(HPSI1013i-aetc_1)
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Bardet-Biedl syndrome
WTSIi034-A
(HPSI0913i-kojv_2)
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Bardet-Biedl syndrome
WTSIi035-A
(HPSI0913i-gooj_1)
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Bardet-Biedl syndrome
WTSIi457-B
(HPSI0115i-iinu_5)
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Bardet-Biedl syndrome
WTSIi527-B
(HPSI0216i-heub_6)
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Usher syndrome
WTSIi474-B
(HPSI0216i-dewh_4)
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Usher syndrome
WTSIi510-B
(HPSI0216i-xanu_6)
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Usher syndrome
WTSIi548-B
(HPSI0416i-mefc_1)
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Usher syndrome
WTSIi399-A
(HPSI0614i-gawh_7)
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WTSIi488-B
(HPSI0516i-oazw_3)
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WTSIi464-B
(HPSI0516i-eitu_4)
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Usher syndrome
WTSIi486-B
(HPSI0516i-hapz_2)
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ZZUi014-A
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Spinocerebellar ataxia type 3
WTSIi149-A
(HPSI0913i-laia_4)
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Bardet-Biedl syndrome
WTSIi150-A
(HPSI0414i-jetz_1)
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Bardet-Biedl syndrome
WTSIi419-A
(HPSI0514i-kidt_1)
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Bardet-Biedl syndrome
WTSIi153-A
(HPSI0414i-puvg_1)
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Bardet-Biedl syndrome
WTSIi422-A
(HPSI0414i-zazi_4)
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Bardet-Biedl syndrome
WTSIi155-A
(HPSI0414i-hutq_1)
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Bardet-Biedl syndrome
WTSIi156-A
(HPSI0414i-xugr_2)
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Bardet-Biedl syndrome
WTSIi157-A
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TRDSi004-A
(TRDS-Cardio-012)
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long QT syndrome 2
WTSIi426-A
(HPSI0714i-mejk_1)
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Bardet-Biedl syndrome
WTSIi160-A
(HPSI0914i-verf_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi162-A
(HPSI0914i-lopq_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi163-A
(HPSI1014i-suok_3)
Donor diseases:
Bardet-Biedl syndrome
WTSIi164-A
(HPSI0614i-foyj_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi166-A
(HPSI0414i-wize_1)
Donor diseases:
Bardet-Biedl syndrome
WTSIi669-B
(HPSI0816i-aorf_2)
Donor diseases:
Usher syndrome
WTSIi453-A
(HPSI0416i-fiwt_3)
Donor diseases:
Usher syndrome
WTSIi457-A
(HPSI0115i-iinu_3)
Donor diseases:
Bardet-Biedl syndrome
Last update 17th February 2023
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Provider

Generator University of Milan (UMIL)

External Databases

BioSamples SAMEA6049742
Cellosaurus CVCL_YC86
Wikidata Q98134197

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Williams-Beuren Syndrome.
Synonyms
  • Fanconi Schlesinger syndrome
  • WBS

External Databases (Donor)

BioSamples SAMEA6049743

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved?
Approval number
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A connective-tissue cell of mesenchymal origin that secretes proteins and especially molecular collagen from which the extracellular fibrillar matrix of connective tissue forms.

Reprogramming method

Vector type Non-integrating
Vector mRNA Reprogramming Kit (Stemgent)

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Medium TeSR™ E8™
Supplements
Pen/Strep 1 %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Unknown

Other Genotyping (Cell Line)