Synonym of WTSIi018-B-1

HPSI0114i-kolf_2-C1

General#

Cell Line

hPSCreg Name WTSIi018-C
Alternative name(s)
HPSI0114i-kolf_2-C1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 22nd May 2019
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Provider

Generator Wellcome Trust Sanger Institute (WTSI)
Owner Wellcome Trust Sanger Institute (WTSI)
Distributors
Derivation country United Kingdom

External Databases

HipSci HPSI0114i-kolf_2-C1
Cellosaurus CVCL_9S58

General Information

Projects
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed
Subclone of

Donor Information#

General Donor Information

Sex male
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes
Non-disease associated phenotypes
  • Healthy

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2398402
HipSci HPSI-kolf

Ethics#

Also have a look at the ethics information for the parental line WTSIi018-B .
Alternatives to consent
Is there an MTA available for the cell line? Yes
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Lifetech
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? Yes
Constraints for use or distribution can only be used for academic reserach not commercial

hIPSC Derivation#

General

The source cell information can be found in the parental cell line WTSIi018-B.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Methods used
Immunostaining

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
No

Culture Conditions#

Feeder cells
No
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
DNMT3B
Yes
GDF3
Yes
nodal
Yes
podxl
Yes
xist
Yes
LEFTY1
Yes
foxd3
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
normal
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes

Genetic Modification#