HPSI0616i-xuvy_6

General#

Cell Line

hPSCreg Name WTSIi524-A
Alternative name(s)
HPSI0616i-xuvy_6
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 9th May 2018
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Provider

Generator Wellcome Trust Sanger Institute (WTSI)
Distributors

External Databases

HipSci HPSI0616i-xuvy_6
BioSamples SAMEA17593168
ECACC 77650646
Cellosaurus CVCL_LM35

General Information

Projects
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex male
Ethnicity White British

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
immune system disease
Synonyms
  • autoimmune diseases
  • Other specified disorders of the immune mechanism (disorder)
  • DEFIC CELL IMMUNITY NOS
  • [X]Disorder involving the immune mechanism, unspecified
  • Other deficiency of cell-mediated immunity
  • Immunodeficiency and Immunosuppression Disorders
  • Other specified disorders involving the immune mechanism
  • Unspecified disorder of immune mechanism
  • Deficiency of cell-mediated immunity
  • Autoimmune disease, not elsewhere classified
  • Immunodeficiency with predominant T-cell defect, unspecified
  • IMMUNDEF T-CELL DEF NOS
  • Disorders involving the immune mechanism
  • AUTOIMMUNE DISEASE NEC
  • Other specified disorders of the immune mechanism
  • Immune System and Related Disorders
  • IMMUNE MECHANISM DIS NOS
  • [X]Disorder involving the immune mechanism, unspecified (disorder)
  • Disorder of the immune mechanism NOS (disorder)
  • IMMUNE MECHANISM DIS NEC
  • Disorder of the immune mechanism NOS
show more synonyms

External Databases (Donor)

BioSamples SAMEA4455862

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 60-64
Collected in 2016
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Notes on reprogramming vector detection CytoTune 2

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
32.919 1.505

Certificate of Analysis

Is there a certificate of analysis available?
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0616i-xuvy_6
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003526
mpileup variant calls
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003526
Raw sequencing reads
RNA-seq
https://ega-archive.org/datasets/EGAD00001003536
Splice-aware STAR alignment
RNA-seq
https://ega-archive.org/datasets/EGAD00001003536
Abundances of transcripts
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003526
BWA alignment