General

Cell Line
hPSCreg name	BCHi027-A-3
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	BCHi027-A-3
Alternative name(s)	HNDS0145-01#A CC3(-/-)
Cell line type	Human induced pluripotent stem cell (hiPSC)
Similar lines	BCHi027-A-1 (HNDS0145-01#A CNC17(+/-)) Donor diseases: Tuberous sclerosis complex BCHi027-A-2 (HNDS0145-01#A CC32-1(+/+)) Donor diseases: Tuberous sclerosis complex BCHi027-A (HNDS0145-01#A) Donor diseases: Tuberous sclerosis complex BCHi026-A-1 (HNDS0136-01#A CNC1(+/-)) Donor diseases: Tuberous sclerosis complex BCHi026-A-2 (HNDS0136-01#A CC20(+/+)) Donor diseases: Tuberous sclerosis complex PFIZi032-A (B214c8) Donor's gene variants: TSC2 Donor diseases: Tuberous Sclerosis BCHi029-A (HNDS0121-01#A) Donor diseases: Tuberous sclerosis complex BCHi029-B (HNDS0121-01#B) Donor diseases: Tuberous sclerosis complex KMUGMCi006-D (TS271 #23 WT) Donor diseases: Tuberous Sclerosis EMCi169-E (Clone_11) Donor diseases: Tuberous sclerosis complex KMUGMCi006-E (TS271 #4 MT) Donor diseases: Tuberous Sclerosis KMUGMCi006-F (TS271 #20 MT) Donor diseases: Tuberous Sclerosis KMUGMCi006-B (TS271 #5 MT) Donor diseases: Tuberous Sclerosis EMCi169-A (Clone 1) Donor diseases: Tuberous sclerosis complex BCHi026-A (HNDS0136-01#A) Donor diseases: Tuberous sclerosis complex EMCi169-B (Clone_3) Donor diseases: Tuberous sclerosis complex EMCi169-C (Clone_9) Donor diseases: Tuberous sclerosis complex EMCi169-D (Clone_4) Donor diseases: Tuberous sclerosis complex KMUGMCi006-A (TS271 #12 WT) Donor diseases: Tuberous Sclerosis KMUGMCi006-C (TS271 #21 WT) Donor diseases: Tuberous Sclerosis WAe009-A-1F (TSC2-/-)
Last update	3rd March 2024
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Provider
Generator	Children's Hospital (BCH)
External Databases
BioSamples	SAMEA115372777
General Information
* Is the cell line readily obtainable for third parties?	Yes Research use: allowed Clinical use: not allowed Commercial use: not allowed
Subclone of	BCHi027-A

Donor Information

General Donor Information
Sex	female
Ethnicity	American, white
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Tuberous sclerosis complex The donor is affected. Synonyms Tuberous sclerosis Bourneville syndrome
Karyotyping (Donor)
Has the donor karyotype been analysed?	No
External Databases (Donor)
BioSamples	SAMEA115372774

Ethics

Also have a look at the ethics information for the parental line BCHi027-A .

Is there an MTA available for the cell line?	No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
The source cell information can be found in the parental cell line BCHi027-A.
Reprogramming method
Vector type	Non-integrating
Vector	Sendai virus
Vector free reprogramming
Other
Derived under xeno-free conditions	Unknown
Derived under GMP?	Unknown
Available as clinical grade?	Unknown

Culture Conditions

Surface coating	Matrigel/Geltrex
Feeder cells	No
Medium	Other medium: Base medium: Stemflex Main protein source: Bovine Serum Albumin (BSA) Serum concentration: 1 %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?	Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?	No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?	Yes

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line BCHi027-A .

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46, XX; no clonal abnormalities Karyotyping method: G-Banding
Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications

Tuberous sclerosis complex Synonyms Tuberous sclerosis Bourneville syndrome Genetic modifications TSC2 (target) Type of modification Isogenic modification Chromosome location Cytoband location:16p13 Nucleotide sequence variant in HGVS format NM_000548.5: c.4594C>T, p,Gln1532* Is the modification homozygous or heterozygous? Homozygous How has the target locus been modified? Changed