Clone_11

General

Cell Line

hPSCreg name EMCi169-E
Cite as:
EMCi169-E (RRID:CVCL_D0VH)
Alternative name(s)
Clone_11
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
EMCi169-A
(Clone 1)
Donor diseases:
Tuberous sclerosis complex
EMCi169-B
(Clone_3)
Donor diseases:
Tuberous sclerosis complex
EMCi169-C
(Clone_9)
Donor diseases:
Tuberous sclerosis complex
EMCi169-D
(Clone_4)
Donor diseases:
Tuberous sclerosis complex
BCHi026-A-1
(HNDS0136-01#A CNC1(+/-))
Donor diseases:
Tuberous sclerosis complex
BCHi026-A-2
(HNDS0136-01#A CC20(+/+))
Donor diseases:
Tuberous sclerosis complex
BCHi027-A-1
(HNDS0145-01#A CNC17(+/-))
Donor diseases:
Tuberous sclerosis complex
BCHi027-A-2
(HNDS0145-01#A CC32-1(+/+))
Donor diseases:
Tuberous sclerosis complex
BCHi027-A-3
(HNDS0145-01#A CC3(-/-))
Donor diseases:
Tuberous sclerosis complex
KMUGMCi006-D
(TS271 #23 WT)
Donor diseases:
Tuberous Sclerosis
KMUGMCi006-E
(TS271 #4 MT)
Donor diseases:
Tuberous Sclerosis
KMUGMCi006-F
(TS271 #20 MT)
Donor diseases:
Tuberous Sclerosis
KMUGMCi006-B
(TS271 #5 MT)
Donor diseases:
Tuberous Sclerosis
BCHi026-A
(HNDS0136-01#A)
Donor diseases:
Tuberous sclerosis complex
KMUGMCi006-A
(TS271 #12 WT)
Donor diseases:
Tuberous Sclerosis
KMUGMCi006-C
(TS271 #21 WT)
Donor diseases:
Tuberous Sclerosis
BCHi027-A
(HNDS0145-01#A)
Donor diseases:
Tuberous sclerosis complex
PFIZi032-A
(B214c8)
Donor's gene variants:
TSC2
Donor diseases:
Tuberous Sclerosis
BCHi029-A
(HNDS0121-01#A)
Donor diseases:
Tuberous sclerosis complex
BCHi029-B
(HNDS0121-01#B)
Donor diseases:
Tuberous sclerosis complex
Last update 14th July 2023
Notes TSC2 variant c.2838-122 G>A
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Provider

Generator Erasmus MC (EMC)
Contact:
Erasmus MC (EMC)

External Databases

BioSamples SAMEA114072284
Cellosaurus CVCL_D0VH
Wikidata Q123031238

General Information

* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex male
Age of donor (at collection) 15-19

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a mosaic carrier for the disease-associated variant.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Tuberous sclerosis
  • Bourneville syndrome

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46, XY
Karyotyping method: Array CGH

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA114004130

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet (publicly visible) : m.ghazvini@erasmusmc.nl
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? EMC METC
Approval number NL60886.078.17
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
1) Reddish in color. 2) relating to erythrocytes or their precursors.
Synonyms
  • Erythroid
Source cell origin
1: The fluid that circulates in the heart, arteries, capillaries, and veins of a vertebrate animal carrying nourishment and oxygen to and bringing away waste products from all parts of the body. 2: A comparable fluid of an invertebrate.
Synonyms
  • whole blood
Age of donor (at collection) 15-19
Collected in 2019

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
RT-PCR
Notes on reprogramming vector detection ct: N/A

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones morphology, marker expression, genotyping
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Stemflex
Main protein source: Albumine
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SOX2
Yes
SSEA-4
Yes
TRA 1-81
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
ZFP42 (REX-1)
Yes
KLF4
Yes
c-MYC
Yes
GDF3
Yes
FGF4
Yes
DPPA5 (ESG1)
Yes
TERT
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
FOXA2
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
KDR
Yes
TBXT (Brachyury)
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
SOX1
Yes
PAX6
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Karyotyping method: Array CGH

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Sequencing
Variant detected in this cell line