BCHi038-A-3

HNDS0165-01#D CC15-20(+/+)

General

Cell Line

hPSCreg name BCHi038-A-3
Cite as:
BCHi038-A-3
Alternative name(s)
HNDS0165-01#D CC15-20(+/+)
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 12th May 2024
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Provider

Generator Children's Hospital (BCH)

External Databases

BioSamples SAMEA115580263

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity White

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is affected.
Synonyms
  • Tuberous sclerosis
  • Bourneville syndrome

External Databases (Donor)

BioSamples SAMEA115527685

Ethics

Also have a look at the ethics information for the parental line BCHi038-A .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line BCHi038-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Medium Other medium:
Base medium: Stemflex
Main protein source: Bovine Albumin Serum (BSA)
Serum concentration: 1 %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line BCHi038-A .

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX; no clonal abnormalities
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Synonyms
  • Tuberous sclerosis
  • Bourneville syndrome
Genetic modifications
TSC2 (target)
Isogenic modification
Cytoband location:16p13
c.4952A>G; p.N1651S
Homozygous
Repaired