GRX-MCiPS4F-A2-ETO2-GLIS2
GENYOi006-A-1
General#
Cell Line |
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| hPSCreg Name | GENYOi006-A-1 |
| Alternative name(s) |
GRX-MCiPS4F-A2-ETO2-GLIS2
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Last update | 15th June 2020 |
| User feedback | |
Provider |
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| Generator | Centre for Genomics and Oncological Research (GENYO) |
| Owner | Centre for Genomics and Oncological Research (GENYO) |
| Derivation country | Spain |
External Databases |
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| BioSamples | SAMEA6438547 |
| Cellosaurus | CVCL_YN83 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information#
General Donor Information |
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| Sex | male |
| Ethnicity | Spaniard Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
46, xy
Karyotyping method:
G-Banding
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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| BioSamples | SAMEA6438546 |
Ethics#
Also have a look at the ethics information for the parental line
GENYOi006-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | Dr. Tanja Gruber (Memphis, USA) |
hIPSC Derivation#
General |
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The source cell information can be found in the parental cell line
GENYOi006-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
| Methods used |
RT-PCR
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Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions#
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
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| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| NANOG |
Yes |
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| SOX2 |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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Differentiation Potency
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46, xy
Karyotyping method:
G-Banding
|
Other Genotyping (Cell Line) |
|
Genetic Modification#
| Disease/phenotype related modifications |
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