iTaf9-11

General#

Cell Line

hPSCreg Name ICAGi001-A
Alternative name(s)
iTaf9-11
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 30th October 2018
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Provider

Generator THE FEDERAL RESEARCH CENTER INSTITUTE OF CYTOLOGY AND GENETICS (ICAG)
Owner THE FEDERAL RESEARCH CENTER INSTITUTE OF CYTOLOGY AND GENETICS (ICAG)
Distributors
Derivation country Russia

External Databases

BioSamples SAMEA5230331
Cellosaurus CVCL_UF64

General Information

Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Mental retardation, autosomal dominant 39; MRD39; OMIM 616521 (chromosome 2p25.3 deletion syndrome)
The donor is a carrier of a disease-associated mutation and affected.
Family history de novo
Is the medical history available upon request? yes, Research Institute of Medical Genetics, TNMRC
Is clinical information available? yes, Research Institute of Medical Genetics, TNMRC

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
arr[GRCh38] 2p25.3(42444_2684871)x1 dn, 2p25.3-p23.3(2771354_24258056)x3 dn
Karyotyping method: Array CGH

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA5230332

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
skin fibroblast
Passage number reprogrammed 5

Reprogramming method

Vector type Integrating
Vector Virus (Lentivirus)
Genes
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
No
Reprogramming vectors silenced?
Unkown
Vector map

Vector free reprogramming

Other

Selection criteria for clones morphology marker expression
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Gelatin
Feeder cells mouse embryonic fibroblasts
Cellfinder Ont Id: EFO_0004040
Passage method Mechanically
CO2 Concentration 5 %
Medium Other medium:
Base medium: DMEM/F12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
bFGF 10 µg/ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
SSEA-3
Yes
Score:
Marker Present Absent
mCpG
OCT4
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
AFP alpha fetoprotein
Yes
sox 17
Yes
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
MSX1
Yes
FLK1
Yes
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
MAP2
Yes
Morphology

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX,der (2)
Karyotyping method: G-Banding

Other Genotyping (Cell Line)