TRPM4
MRIi017-A
General
Cell Line |
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| hPSCreg name | MRIi017-A |
| Cite as: | MRIi017-A (RRID:CVCL_A0TB) |
| Alternative name(s) |
TRPM4
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
HDZi003-A (hiPSC NP0038) Donor's gene variants: TMEM43 Donor diseases: arrhythmogenic right ventricular dysplasia 5 |
| Last update | 8th June 2023 |
| User feedback | |
Provider |
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| Generator |
Klinikum rechts der Isar (MRI)
Contact:
Klinikum rechts der Isar (MRI) |
External Databases |
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| BioSamples | SAMEA9468731 |
| Cellosaurus | CVCL_A0TB |
| Wikidata | Q108820966 |
General Information |
|
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Donor Information
General Donor Information |
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| Sex | unknown |
Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed.
|
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
Unknown
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External Databases (Donor) |
|
| BioSamples | SAMEA9468732 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | Controlled Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Ethik Kommission, Fakultät für Medizin, Klinikum rechts der Isar (TUM) |
| Approval number | 2109/08, Teilprojekt 4 |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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| Source cell type | |
| Source cell origin |
An organ that constitutes the external surface of the body. It consists of the epidermis, dermis, and skin appendages.
Synonyms
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
| Methods used |
RT-PCR
|
| Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex | |||||||||
| Feeder cells |
No |
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| Passage method |
Enzymatically
Dispase
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| CO2 Concentration | 5 % | |||||||||
| Medium |
Essential 8™
Supplements
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
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| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
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| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| TRA 1-81 |
Yes |
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| NANOG |
Yes |
Differentiation Potency
In vitro spontaneous differentiation
| Marker | Expressed |
| SOX7 SRY-box transcription factor 7 |
Unknown |
Protocol or reference
In vitro spontaneous differentiation
| Marker | Expressed |
| ACTA2 – actin alpha 2, smooth muscle |
Unknown |
Protocol or reference
In vitro spontaneous differentiation
| Marker | Expressed |
| KRT14 keratin 14 |
Unknown |
Protocol or reference
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Unknown
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Other Genotyping (Cell Line) |
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