HPSI0316i-muwv_3

General#

Cell Line

hPSCreg Name WTSIi448-A
Alternative name(s)
HPSI0316i-muwv_3
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 9th May 2018
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Wellcome Trust Sanger Institute (WTSI)
Distributors

External Databases

HipSci HPSI0316i-muwv_3
BioSamples SAMEA4448534
ECACC 77650502
Cellosaurus CVCL_IX77

General Information

Projects
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex male
Ethnicity Asian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Rare hereditary ataxia

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA4339709

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 40-44
Collected in 2016
Source cell line vendor University College London

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Notes on reprogramming vector detection CytoTune 2

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
22.325 1.479

Certificate of Analysis

Is there a certificate of analysis available?
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0316i-muwv_3
Number of regions different from primary tissue: 1; Length of differences from primary tissue: 1
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003521
BWA alignment
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003521
Raw sequencing reads
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003521
mpileup variant calls
RNA-seq
https://ega-archive.org/datasets/EGAD00001003538
Splice-aware STAR alignment
RNA-seq
https://ega-archive.org/datasets/EGAD00001003538
Abundances of transcripts