XLC-348

General#

Cell Line

hPSCreg Name CBi001-A
Alternative name(s)
XLC-348
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 22nd May 2019
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Provider

Generator Creative Bioarray (CB)
Distributors
Derivation country United States

External Databases

BioSamples SAMEA4458267
Cellosaurus CVCL_IT53

General Information

Projects
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: allowed
Commercial: allowed

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 20-24
Ethnicity Caucasian, Jewish

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
schizophrenia
The donor is affected.
Synonyms
  • Other specified types of schizophrenia, unspecified state
  • SCHIZOPHRENIA NEC-UNSPEC
  • Schizophrenia (disorder)
  • SCHIZO NEC-CHR/EXACERB
  • Unspecified schizophrenia (disorder)
  • Disorder, Schizophrenic
  • Other specified types of schizophrenia, chronic state
  • SCHIZO NEC-SUBCHR/EXACER
  • Schizophrenia, NOS
  • [X]Schizophrenia, unspecified
  • Unspecified schizophrenia, chronic state with acute exacerbation
  • schizophrenia-1
  • Unspecified schizophrenia, subchronic state with acute exacerbation
  • Schizophrenic Disorder
  • Schizophrenia NOS (disorder)
  • Schizophrenic Disorders
  • Other specified types of schizophrenia, subchronic state with acute exacerbation
  • Schizophrenia NOS
  • Dementia Praecox
  • SCHIZO NOS-CHR/EXACERB
  • Other specified types of schizophrenia, subchronic state
  • Unspecified schizophrenia, unspecified state
  • Other specified types of schizophrenia
  • Schizophrenic disorders (disorder)
  • Other specified types of schizophrenia, in remission
  • SCHIZOPHRENIA NEC-SUBCHR
  • SCHIZOPHRENIA NEC-CHR
  • SCHIZOPHRENIC DIS
  • Schizophrenias
  • [X]Schizophrenia, unspecified (disorder)
  • Unspecified schizophrenia
  • SCHIZOPHRENIA NEC-REMISS
  • SCHIZOPHRENIA NOS-UNSPEC
  • Disorders, Schizophrenic
  • SCHIZO NOS-SUBCHR/EXACER
  • Other specified types of schizophrenia, chronic state with acute exacerbation
show more synonyms
Disease associated phenotypes
  • paralogical thinking
  • affective shielding
  • splitting of affect from content
  • suspiciousness
Family history positive family history
Is the medical history available upon request? no
Is clinical information available? no

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
47,XY,+mar[1]/46,XY[24]

External Databases (Donor)

BioSamples SAMEA4458266

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet:
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? No
If you do not hold the SIGNED Donor Consent Form, do you know who does? Yes
Contact information / weblink
Alternatives to consent
Alternative consent approval number
Has the donor agreed to be re-contacted? No
Has the donor been informed about how her/his data will be protected? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Details on restriction to research project
Does consent permit unforeseen future research, without further consent? No
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? No
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? No
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes
an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? No
Policy for access to genetic information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Please describe how access is provided:
Contact data, institution, or website:
Name of accrediting authority involved?
Approval number
Name of accrediting authority involved?
Approval number
Please describe:
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
Further constraints on use
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Constraints for use or distribution

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Age of donor (at collection) 20-24

Reprogramming method

Vector type Integrating

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Medium Other medium:
Base medium: DMEM/F12
Main protein source: Knock-out serum replacement
Serum concentration: %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
SOX2
Yes
POU5F1 (OCT-4)
Yes
DNMT3B
Yes
TERT
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
In vitro directed differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
47,XY,+mar[1]/46,XY[24]

Other Genotyping (Cell Line)