WA16

General

Cell Line

hPSCreg name WISCe002-A
Cite as:
WISCe002-A (RRID:CVCL_9777)
Alternative name(s)
WA16
Cell line type Human embryonic stem cell (hESC)
Similar lines
KAUSTi009-A
(KS3-iPSC#A)
Donor diseases:
Klinefelter's syndrome
KAUSTi009-B
(KS3-iPSC#B)
Donor diseases:
Klinefelter's syndrome
KAUSTi010-A
(KS5-iPSC#A)
Donor diseases:
Klinefelter's syndrome
KAUSTi001-B
(KS7-iPSC#B)
Donor diseases:
Klinefelter's syndrome
KAUSTi010-B
(KS5-iPSC#B)
Donor diseases:
Klinefelter's syndrome
KAUSTi006-A
(KS6-iPSC#A)
Donor diseases:
Klinefelter's syndrome
KAUSTi006-B
(KS6-iPSC#B)
Donor diseases:
Klinefelter's syndrome
KAUSTi003-A
(KS1-iPSC#D)
Donor diseases:
Klinefelter's syndrome
KAUSTi008-B
(KS4-iPSC#B)
Donor diseases:
Klinefelter's syndrome
KAUSTi008-C
(KS4-iPSC#C)
Donor diseases:
Klinefelter's syndrome
KAUSTi008-D
(KS4-iPSC#D)
Donor diseases:
Klinefelter's syndrome
KAUSTi008-E
(KS4-iPSC#E)
Donor diseases:
Klinefelter's syndrome
KAUSTi007-A
(KS2-iPSC#A)
Donor diseases:
Klinefelter's syndrome
KAUSTi008-F
(KS4-iPSC#F)
Donor diseases:
Klinefelter's syndrome
KAUSTi008-G
(KS4-iPSC#G)
Donor diseases:
Klinefelter's syndrome
KAUSTi008-A
(KS4-iPSC#A)
Donor diseases:
Klinefelter's syndrome
KAUSTi001-A
(KS7-iPSC#A)
Donor diseases:
Klinefelter's syndrome
KAUSTi007-B
(KS2-iPSC#B)
Donor diseases:
Klinefelter's syndrome
KCLe007-A
(KCL008_HD2)
Donor diseases:
Klinefelter syndrome
RIe004-A
(Royan H4)
Donor diseases:
Klinefelter syndrome
CEBe001-A
(FC018)
Donor diseases:
Klinefelter syndrome
Last update 4th September 2022
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator University of Wisconsin (WISC)
Derivation country United States

External Databases

Cellosaurus CVCL_9777
NIHhESC NIHhESC-11-0097
Wikidata Q54993519

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is affected.
Synonyms
  • Hypogonadotropic Hypogonadism
  • Klinefelter's syndrome
  • XXY syndrome
  • XXY trisomy
  • 47, XXY
show more synonyms

Ethics

Alternative consent approval number NIHhESC-11-0097
How may genetic information associated with the cell line be accessed?

hESC Derivation

Embryo stage Blastula with ICM and Trophoblast
ZP removal technique Enzymatic
Cell isolation Mechanical

Culture Conditions

Surface coating Matrigel/Geltrex
Passage method Mechanically
O2 Concentration 5 %
CO2 Concentration 10 %
Medium mTeSR™ 1
Supplements
Human Serum Albumin
bFGF
GABA
LiCl
Serum Replacement

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
ZFP42 (REX-1)
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
47XXY

Other Genotyping (Cell Line)