HPSI0414i-eevy_6

General

Cell Line

hPSCreg name WTSIi195-A
Cite as:
WTSIi195-A (RRID:CVCL_AJ87)
Alternative name(s)
HPSI0414i-eevy_6
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
WTSIi195-B
(HPSI0414i-eevy_7)
Donor diseases:
monogenic diabetes
WTSIi204-A
(HPSI0414i-oulr_2)
Donor diseases:
monogenic diabetes
WTSIi635-A
(HPSI0414i-zabk_4)
Donor diseases:
monogenic diabetes
WTSIi204-B
(HPSI0414i-oulr_4)
Donor diseases:
monogenic diabetes
WTSIi192-A
(HPSI0414i-biiw_1)
Donor diseases:
monogenic diabetes
WTSIi193-A
(HPSI0414i-cekz_1)
Donor diseases:
monogenic diabetes
WTSIi196-A
(HPSI0514i-eoxi_6)
Donor diseases:
monogenic diabetes
WTSIi200-A
(HPSI0414i-kefk_2)
Donor diseases:
monogenic diabetes
WTSIi202-A
(HPSI0714i-oebj_1)
Donor diseases:
monogenic diabetes
WTSIi203-A
(HPSI0714i-oojs_1)
Donor diseases:
monogenic diabetes
WTSIi436-A
(HPSI0714i-zaos_1)
Donor diseases:
monogenic diabetes
WTSIi438-A
(HPSI0714i-pirs_1)
Donor diseases:
monogenic diabetes
WTSIi439-A
(HPSI0714i-mitg_4)
Donor diseases:
monogenic diabetes
WTSIi445-A
(HPSI0714i-xebt_2)
Donor diseases:
monogenic diabetes
WTSIi194-A
(HPSI0614i-dixh_2)
Donor diseases:
monogenic diabetes
WTSIi197-A
(HPSI0714i-fasu_2)
Donor diseases:
monogenic diabetes
WTSIi199-A
(HPSI0614i-guyj_2)
Donor diseases:
monogenic diabetes
WTSIi409-A
(HPSI0414i-walu_1)
Donor diseases:
monogenic diabetes
WTSIi475-A
(HPSI0514i-qajt_5)
Donor diseases:
monogenic diabetes
WTSIi205-A
(HPSI0514i-pulk_1)
Donor diseases:
monogenic diabetes
WTSIi416-A
(HPSI0514i-aomr_5)
Donor diseases:
monogenic diabetes
WTSIi475-B
(HPSI0514i-qajt_2)
Donor diseases:
monogenic diabetes
WTSIi416-B
(HPSI0514i-aomr_6)
Donor diseases:
monogenic diabetes
WTSIi424-A
(HPSI0514i-mure_9)
Donor diseases:
monogenic diabetes
WTSIi424-B
(HPSI0514i-mure_4)
Donor diseases:
monogenic diabetes
WTSIi431-A
(HPSI0514i-tavh_2)
Donor diseases:
monogenic diabetes
WTSIi410-B
(HPSI0614i-zoio_1)
Donor diseases:
monogenic diabetes
WTSIi417-B
(HPSI0614i-voxu_2)
Donor diseases:
monogenic diabetes
WTSIi444-A
(HPSI0714i-riya_2)
Donor diseases:
monogenic diabetes
WTSIi396-A
(HPSI0414i-kodf_2)
Donor diseases:
monogenic diabetes
WTSIi398-A
(HPSI0414i-rauj_1)
Donor diseases:
monogenic diabetes
WTSIi400-A
(HPSI0414i-tout_1)
Donor diseases:
monogenic diabetes
WTSIi401-A
(HPSI0714i-meet_3)
Donor diseases:
monogenic diabetes
WTSIi198-A
(HPSI0414i-fuai_1)
Donor diseases:
monogenic diabetes
WTSIi408-A
(HPSI0414i-niim_3)
Donor diseases:
monogenic diabetes
WTSIi408-B
(HPSI0414i-niim_5)
Donor diseases:
monogenic diabetes
WTSIi201-A
(HPSI0614i-koqx_1)
Donor diseases:
monogenic diabetes
WTSIi410-A
(HPSI0614i-zoio_2)
Donor diseases:
monogenic diabetes
WTSIi477-A
(HPSI0514i-kooz_5)
Donor diseases:
monogenic diabetes
WTSIi417-A
(HPSI0614i-voxu_1)
Donor diseases:
monogenic diabetes
WTSIi430-B
(HPSI0514i-aecv_3)
Donor diseases:
monogenic diabetes
WTSIi428-A
(HPSI0714i-lutt_6)
Donor diseases:
monogenic diabetes
WTSIi430-A
(HPSI0514i-aecv_2)
Donor diseases:
monogenic diabetes
Last update 31st October 2019
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Provider

Generator Wellcome Sanger Institute (WTSI)
Distributors
Derivation country United Kingdom

External Databases

BioSamples SAMEA3848091
HipSci HPSI0414i-eevy_6
EBiSC WTSIi195-A
Cellosaurus CVCL_AJ87
Wikidata Q54890700

General Information

Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male
Age of donor (at collection) 15-19
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • monogenic diabetes mellitus
  • monogenic diabetes
  • rare genetic diabetes mellitus

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2464817
HipSci HPSI-eevy

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. Andrew Hattersley, University of Exeter medical school
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information http://www.hipsci.org/model-ethics-forms
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? No
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? No
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
Does consent expressly permit collection of genetic information? Yes
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? Yes
How may genetic information associated with the cell line be accessed?
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? No
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? No
Does consent permit access to medical records of the donor? Yes
Please describe how access is provided:
Does consent permit access to any other source of information about the clinical treatment or health of the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? NRES Committee Yorkshire & The Humber - Leeds West
Approval number 08/H0713/82
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A leukocyte with a single non-segmented nucleus in the mature form.
Synonyms
  • mononuclear leukocyte
  • peripheral blood mononuclear cell
Source cell origin
A fluid that is composed of blood plasma and erythrocytes.
Synonyms
  • portion of blood
  • vertebrate blood
  • whole blood
Age of donor (at collection) 15-19
Collected in 2015
Source cell line vendor University of Exeter Medical School

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
32.901 1.375

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 20

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0414i-eevy_6
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
RNA-seq
https://ega-archive.org/datasets/EGAD00001003530
Abundances of transcripts
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
Raw sequencing reads
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
mpileup variant calls
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001149
Text file with probe intensities
RNA-seq
https://ega-archive.org/datasets/EGAD00001003530
Splice-aware STAR alignment
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
BWA alignment
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001149
Array signal intensity data