HPSI0414i-kodf_2

General#

Cell Line

hPSCreg Name WTSIi396-A
Alternative name(s)
HPSI0414i-kodf_2
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 9th May 2018
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Provider

Generator Wellcome Trust Sanger Institute (WTSI)
Distributors

External Databases

HipSci HPSI0414i-kodf_2
BioSamples SAMEA2708758
ECACC 77650556
Cellosaurus CVCL_AF20

General Information

Projects
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
monogenic diabetes
Synonyms
  • monogenic diabetes mellitus

External Databases (Donor)

BioSamples SAMEA2464783

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 20-24
Collected in 2014
Source cell line vendor University of Exeter Medical School

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
28.87 1.52

Certificate of Analysis

Is there a certificate of analysis available?
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0414i-kodf_2
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Genotyping array
https://ega-archive.org/datasets/EGAD00010000779
Genotyping array calls
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
BWA alignment
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
Raw sequencing reads
Genotyping array
https://ega-archive.org/datasets/EGAD00010000779
Imputed and phased genotypes
Genotyping array
https://ega-archive.org/datasets/EGAD00010000779
Array signal intensity data
RNA-seq
https://ega-archive.org/datasets/EGAD00001003530
Abundances of transcripts
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
mpileup variant calls
RNA-seq
https://ega-archive.org/datasets/EGAD00001003530
Splice-aware STAR alignment