HPSI0414i-oulr_4

The cell line is not validated yet.

General#

Cell Line

hPSCreg Name WTSIi204-B
Alternative name(s)
HPSI0414i-oulr_4
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 9th May 2018
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Provider

Generator Wellcome Trust Sanger Institute (WTSI)
Distributors

External Databases

BioSamples SAMEA3847991
ECACC 77650356
HipSci HPSI0414i-oulr_4
Cellosaurus CVCL_AJ91

General Information

Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
monogenic diabetes
Synonyms
  • monogenic diabetes mellitus

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2464827
HipSci HPSI-oulr

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
mononuclear cell
A leukocyte with a single non-segmented nucleus in the mature form.
Synonyms
  • mononuclear leukocyte
Source cell origin
blood
A fluid that is composed of blood plasma and erythrocytes.
Synonyms
  • portion of blood
  • vertebrate blood
Age of donor (at collection) 15-19
Collected in 2015
Source cell line vendor University of Exeter Medical School

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
33.959 1.338

Certificate of Analysis

Is there a certificate of analysis available?
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0414i-oulr_4
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
RNA-seq
https://ega-archive.org/datasets/EGAD00001003530
Abundances of transcripts
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
mpileup variant calls
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
BWA alignment
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
Raw sequencing reads
RNA-seq
https://ega-archive.org/datasets/EGAD00001003530
Splice-aware STAR alignment
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001149
Array signal intensity data
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001149
Text file with probe intensities